NGAL、MMP-9在食管癌中的表达及其临床意义

Expression and clinical significance of NGAL and MMP-9 in human esophageal carcinoma

目的探讨中性粒细胞明胶酶相关脂质运载蛋白(NGAL)和基质金属蛋白酶9(MMP-9)在食管癌中的表达及二者与食管癌之间的相关性。方法用免疫组织化学ABC法检测58例食管癌组织及18例正常食管组织中的NGAL和MMP-9的表达。结果 NGAL、MMP-9在食管癌中的表达显著高于正常组织,差异有统计学意义(P<0.05);NGAL和MMP-9与肿瘤患者的性别、年龄及肿瘤的组织学类型无相关性(P>0.05),与肿瘤的分化程度、浸润深度及淋巴结转移相关(P<0.05);NGAL和MMP-9在食管癌中的表达存在关联性(r=0.572,P<0.05)。结论 NGAL与MMP-9在食管癌中明显异常高表达,且在食管癌的发生、浸润及与淋巴结转移过程中关系密切并可能存在协同作用,两者可作为食管癌侵袭转移及预后的重要指标。

Objective To investigate the expression and clinical significance of neutrophil gelatinase asso- ciated lipocalin （NGAL） and matrix metalloproteinases-9 （MMP-9） in human esophageal carcinoma. Methods ABC immunohistochemieal method was carried out to detect the NGAL and MMP-9 protein among 18 normal tissues and 58 cancer tissues. Results The expression of NGAL, MMP-9 in the cancer group are obviously higher than the normal group（ P 〈 0. 05 ） , NGAL and MMP-9 played an important role in infiltration, lymph node metastasis and carcinomatous changes of mucosal epithelium in esophageal carcinoma （ P 〈 0. 05 ） ; there was a correlation between expression of NGAL and MMP-9 in esophageal carcinoma（ r = 0. 572, P 〈 0. 05 ）. Conclusions The expression of NGAL and MMP-9 is increased in the tissue of esophageal carcinoma and is related to the occurrence, development and metastasis of esophageal cancer. Combined detecting of NGAL and MMP-9 may play an important role in judging the invasion, metastasis and prognosis for esophageal carcinoma.