丹参对大鼠粘连成纤维细胞增殖与胶原合成的作用研究

Effect of Danshen on proliferation and collagen synthesis of fibroblasts from rat adhesions

目的:研究中药丹参对大鼠粘连成纤维细胞增殖与胶原合成的影响。方法:利用原代培养获得大鼠粘连组织成纤维细胞(AFB),以地塞米松为阳性对照药,考察丹参对AFB增殖活性与培养液羟脯氨酸含量(HyP)的影响。结果:随着剂量增加,丹参组AFB活性降低趋势大于地塞米松组,丹参组的IC50也显著低于地塞米松组(P<0.01);丹参各剂量组与地塞米松组的HyP水平均低于空白对照组,存在显著性差异(P<0.01);丹参各剂量组与地塞米松组的HyP水平无显著性差异。结论:抑制AFB增殖,减少其胶原合成是丹参防治术后粘连的作用机制。

Objective：To study the effect of Danshen on proliferation and collagen synthesis of cultured fibroblasts from rat adhesions.Method：Adhesive fibroblasts（AFB） of rats were harvested by primary culture.Dexamethasone was used as positive control.Proliferation of AFB and hydroxyproline（HyP） content in culture fluid were detected.Result：Following the increase of drug dosage,AFB viability decreasing tendency of Danshen groups was higher than dexamethasone group.The IC50 of Danshen on AFB was significantly lower than dexamethasone（P0.01）;HyP level of Danshen groups and dexamethasone group were significantly lower than blank control（P0.01）.But there was no significant difference between Danshen groups and dexamethasone group.Conclusion：To inhibit the proliferation and decrease collagen synthesis of AFB was the preventive mechanism of Danshen on post-surgical adhesion.