上消合剂对大鼠急性放射性食管损伤时外周血细胞及炎性因子影响的实验研究

Experimental study on impact of Shangxiao Decoction to peripheral blood cell and inflammatory factor of radiation esophageal injury of rats

目的:通过观察中药上消合剂(SXD)对大鼠急性放射性食管损伤中外周血细胞及炎性因子的影响了解其防治机制。方法:将100只雄性SD大鼠随机均分为空白组、造模组、生理盐水组、西药组及中药上消合剂组。用6MV-X射线对大鼠上段食管单次照射43Gy,分别于照射后第7天及第14天各组随机抽取10只大鼠取血,检测血细胞及炎性因子IL-1、IL-6、TNF-α的水平。结果:造模后各组大鼠出现饮水、进食量减少及体重下降等放射性食管炎的表现。与造模组相比,上消合剂组一般状况改善,外周血淋巴细胞相对增高(P<0.05),IL-1、TNF-α的表达及中性粒细胞相对降低(P<0.05)。结论:中药上消合剂可减轻急性放射性食管损伤。作用机制可能与抑制炎性因子的表达、促进淋巴细胞恢复有关。

Objective：To observe the effect of Shangxiao Decoction to peripheral blood cell and inflammatory factor of esophageal injury of rats and to investigate the mechanism.Methods：One hundred SD male rats were randomly divided into 5 groups：blank control group,model group,saline group,western medicine group and Shangxiao Decoction group.The upper paragraph esophaguses of all rats except those in the blank group were given single doses of radiation of 43Gy by 6MV-X ray.The changes of physiological conditions after radiation were investigated.Ten rats in each group were randomly killed humanely on the 7th and 14th day after irradiation.The serum inflammation-inducing factors＇ levels of IL-1,IL-6,TNF-α and the leucocyte count and different in Peripheral blood were detected.Results：After irradiation,the rats presented the performance of the acute radiotherapy-induced esophagitis such as intaking water and feed＇s reducing and body weight＇s dropping.Shangxiao Decoction could improved the whole conditions.Compared with the model group,the count of peripheral blood lymphocyte in the Shangxiao Decoction group was higher（P0.05）.The levels of IL-1,TNF-α and neutrophil were lower in the Shangxiao Decoction group than in the model group（P0.05）.Conclusion：Traditional chinese medicine shang xiao decoction can reduce the acute radiation esophageal injury and its mechanism may be relevant to inhibiting the expression of inflammation-inducing factors,promoting lymphocyte recovery.