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腹侧海马内强啡肽与癫痫发作敏感性形成关系探讨

A Study on the relationships between dynorphin in the ventral hippocampus and seizure susceptibility
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摘要 用免疫组化方法检测一次给予惊厥剂量海人酸(Kanicacid, KA) 诱发SD大鼠出现急性癫痫发作后, 癫痫发作敏感性形成过程中(KA 后1~7 d) ,海马内内源性强啡肽A(1- 8) 免疫反应活性(DYNA (1- 8) - LI) 动态变化过程。结果表明, KA后1~7 d, 背、腹侧海马苔状纤维(MF) DYNA(1-8) 明显增强, KA后2~4 d, 在腹侧海马齿状回颗粒细胞(DGCs) 层部位出现DYNA(1-8) -LI免疫反应阳性神经元,5~7 d 后上述改变逐渐消失。研究中另一组动物被秋水仙素(Colchicine, 简称col-) 1-5 μg/0-6 μl 选择性分别或同时破坏双侧背侧和腹侧海马DGCs, 用以删除海马内源性强啡肽的作用,7d 后, 用8 mg/kg KA检测其对癫痫刺激的敏感性, 结果显示, col- 损伤双侧腹侧海马DGCs 组动物, 可使KA诱发的癫痫活动明显加重。表明一次KA后,腹侧海马DGCs 部位DYNA(1-8) - LI一过性高表达很可能与5 ~7 d 前癫痫发作敏感性不能形成有关。 The dynamic changing process of the immuno-reactivities of endogenous dynorphin A(1-8) in hippocampus of the rat during the formation process of seizure susceptibility(1~7 d after KA) was investigated after acute seizure induced by single systemic administration of convulsive dose of kinate acid(KA)by immunohistochemistry in this study.The result showes that the immunoreactivity of DYN was obviously increased in both dosal and ventral hippocampal mossy fibres 1~7 d after KA treatment.And DYN A(1-8)immunareactive neurons can be seen among dentate granular cells in ventral hippocampus 2~4 d after KA treatment.Theses changes gradually disappeared 5~7 d after KA treatment.In order to avoid the actions of endogenous DYN,another group of animals was selectivelly injected colchicine (1 5 μg/μl) both or seperately into dorsal and/or ventral hippocampus of both sides.The animals were injected KA(8 mg/kg) 7d after colchicine treatment.The result showes that,KA induced seizure activities were higher in those animals which were injected colchicine than those animals which were injected NS into ventral hippocampus.This suggests that the short period high expression of DYN A(1-8) in the ventral hippocmapal dentate granular cells might account for the failure of the induction of seizure susceptibility within 5~7 d after KA treatment.
出处 《大连医科大学学报》 CAS 1999年第4期249-253,共5页 Journal of Dalian Medical University
关键词 海人酸 海马 癫痫发作敏感性 强啡肽 癫痫 kainic acid hippocampus seizure susceptibility dynorphin
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