利开灵对脑出血大鼠继发肠源性内毒素血症的干预作用

Intervention Effect of Likailing Decoction on Secondary Intestinal Endotoxemia After Intracerebral Hemorrhage in Rats

目的:探讨利开灵对脑出血后大鼠体内继发肠源性内毒素血症的干预作用。方法:采用Rosenberg法改良后建立大鼠脑出血模型,根据脑出血不同时点(12h、24h、48h和72h)分为4组,每个时间点再分为3组:假手术组、模型组、利开灵组,观察利开灵对肠组织相应病理改变的影响,并用终点显色法检测各组血浆内毒素(LPS)水平,ELISA法检测各组血清TNF-α、IL-1β水平。结果:模型组血浆LPS各时点均明显升高,在48h达高峰(P<0.01);血清TNF-α、IL-1β升高,均在24h达高峰(P<0.01)。同时发现利开灵可明显改善肠组织病理形态损伤,下调血浆LPS以及血清TNF-α、IL-1β过高表达。结论:脑出血大鼠继发肠源性内毒素血症,利开灵对其有较好的调控作用,这对改善脑出血预后具有积极意义。

Objective：To explore the intervention effect of Likailing Decoction on secondary intestinal endotoxemia after intracerebral hemorrhage in rats.Methods：Rat models of intracerebral hemorrhage were established by improved Rosenberg method.Rats were randomly divided into sham group,model group and LKL treatment group.Observing the the effect of Likailing Decoction on pathological morphology changes of intestine,the LPS content level in the plasma by endpoint chromogenic assay,and the TNF-α,IL-1βcontent level in the serum by ELISA at 12,24,48,72 hours.Results：The LPS content in the plasma of model group rats obviously increased and reach the peak at 48h（P0.01）,and the TNF-α,IL-1βcontent in the serum also obviously increased and reach the peak at 24h（P0.01）.Likailing Decoction has the effect on lightening the injury degree on pathological morphology of intestine,and could reduce the high expression of LPS in the plasma and TNF-α,IL-1βin the serum.Conclusion：Intracerebral hemorrhage may cause intestinal endotoxemia in rats.Likailing Decoction has regulatory effect on this,which contribute to the prognosis of intracerebral hemorrhage.