急性低氧对小鼠黏附分子CHL1组织水平表达的影响

Influence of acute hypoxia on CHL1 expression in different tissues of mice

目的:观察急性低氧条件下小鼠脑组织及心、肺、肾中黏附分子CHL1的表达变化,为探讨黏附分子CHL1在低氧损伤中的调节作用提供依据。方法:利用本室已建立的小鼠急性低氧模型,将雄性成年C57BL/6小鼠随机分为低氧处理组和常氧对照组(n=10)。低氧8 h后提取组织蛋白,通过Western blot检测急性低氧处理后CHL1在脑组织和心、肺、肾中的表达变化。结果:CHL1广泛表达于中枢神经系统,急性低氧处理后,CHL1在小鼠脑组织的皮层、下丘脑、脑干中表达明显下调,而在小脑组织中的表达明显上调;CHL1在心、肺、肾中有少量的表达,急性低氧处理后CHL1蛋白在心脏和肺中的表达则下调。结论:黏附分子CHL1在急性低氧刺激后,在脑、心、肺等部位中表达均有明显的改变,提示其在机体低氧损伤过程中可能起重要的调节作用。

Objective： To observe the effects of acute hypoxia on the cell adhesion molecule close homologue of L1 （ CHL1 ） expression in different brain areas and main organs（heart, hmg, kidney）of mice, and provide a basis for the role of CHL1 in hypoxia injury. Methods： Mice were randomly divided into two groups（ n = 10）： normoxia group and hypoxia group. Hypoxia group were treated by acute hypoxia （8% O2, 8 h）. Protein expression changes in different tissues were evaluated by Western blot. Results： In central nervous system, CHL1 protein expressions were down-regulated in cerebral cortex, hypothalamus and brain stem by acute hypoxia and up-regulated in cerebellum. In heart and lung, CHL1 protein expression were down-regulated by acute hypoxia. Conclusion： CHL1 protein expressions were changed in different tissues after acute hypoxia, which suggested CHL1 might play an important role in hypoxia damage regulation.