摘要
目的:探讨髓过氧化物酶129A/G基因多态性与冠心病严重程度关系。方法:入选住院冠心病患者267例,均行冠脉造影检查。用比色法测定血清髓过氧化物酶活性;用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)确定各研究对象的基因型。根据冠脉造影结果分别采用冠脉病变支数和Gensini评分来评估冠心病严重程度。结果:MPO129位点A、G等位基因频率分别为0.107和0.893。不同基因型组间血清髓过氧化物酶活性无明显差异,不同基因型在Gensini评分组间的分布无明显差异,基因型GG患者冠脉病变倾向于多血管病变。结论:髓过氧化物酶129A/G基因多态性与血清髓过氧化物酶活性无显著相关,其不能作为预测冠心病病变严重程度的理想指标。
Objective: To explore the relationship between myeloperoxidase (MPO) 129 A/G promoter polymorphisms and the severity of coronary artery disease (CAD). Me/hods: The study enrolled 267 patients who had been diagnosed as coronary artery diseases by coronary angiography. The senun MPO activity was detected by colorimetric method. PCR-RFLP method was used to decide the genotypes of the patients. The severity of CAD was evaluated by the numbers of stenotic coronary arteries and the Gensini scores respectively. Results: The MPO 129 locus G and A alleles frequency were 0.893 and 0.107, respectively. No significant difference was observed in serum MPO activity between different genotypes ( P 〉 0.05). The distribution of genotypes in different Gensini score groups had no significant difference ( P 〉 0.05). The pa- tients with GG genotypes were prone to develop mutivessel diseases. Conclusion: No significant correlation exists in MPO 129 locus polymorphism and serum MPO activity. The MPO 129 locus pelymorphism isn't a reasonable predict factor of CAD severity.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2011年第3期306-310,共5页
Chinese Journal of Applied Physiology
基金
天津市自然科学基金课题资助(09JCYBJC11900)