MPO129A/G基因多态性与冠心病严重程度相关研究

Correlation between MPO 129 A/G polymorphism and severity of coronary artery disease

目的:探讨髓过氧化物酶129A/G基因多态性与冠心病严重程度关系。方法:入选住院冠心病患者267例,均行冠脉造影检查。用比色法测定血清髓过氧化物酶活性;用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)确定各研究对象的基因型。根据冠脉造影结果分别采用冠脉病变支数和Gensini评分来评估冠心病严重程度。结果:MPO129位点A、G等位基因频率分别为0.107和0.893。不同基因型组间血清髓过氧化物酶活性无明显差异,不同基因型在Gensini评分组间的分布无明显差异,基因型GG患者冠脉病变倾向于多血管病变。结论:髓过氧化物酶129A/G基因多态性与血清髓过氧化物酶活性无显著相关,其不能作为预测冠心病病变严重程度的理想指标。

Objective： To explore the relationship between myeloperoxidase （MPO） 129 A/G promoter polymorphisms and the severity of coronary artery disease （CAD）. Me/hods： The study enrolled 267 patients who had been diagnosed as coronary artery diseases by coronary angiography. The senun MPO activity was detected by colorimetric method. PCR-RFLP method was used to decide the genotypes of the patients. The severity of CAD was evaluated by the numbers of stenotic coronary arteries and the Gensini scores respectively. Results： The MPO 129 locus G and A alleles frequency were 0.893 and 0.107, respectively. No significant difference was observed in serum MPO activity between different genotypes （ P 〉 0.05）. The distribution of genotypes in different Gensini score groups had no significant difference （ P 〉 0.05）. The pa- tients with GG genotypes were prone to develop mutivessel diseases. Conclusion： No significant correlation exists in MPO 129 locus polymorphism and serum MPO activity. The MPO 129 locus pelymorphism isn＇t a reasonable predict factor of CAD severity.