摘要
目的:小鼠心肌梗死模型制备中采用改良气管插管方法,提高模型制备成功率。方法:小切口暴露小鼠气管,直视下进行经口气管插管,左冠状动脉前降支结扎制备心肌梗死模型,观察、记录小鼠心脏颜色和心电图、术后14 d存活情况和心肌组织病理学变化。结果:采用改良气管插管,成功完成40只小鼠心肌梗死模型制作,均可见冠状动脉结扎后,心室前壁颜色变暗,心电图Ⅱ导联ST段明显抬高。除去手术过程中意外死亡,术后14 d存活27只,心肌梗死小鼠实际成活率达到87.1%。开胸后,肉眼可见模型组小鼠左室心腔明显扩大,心室壁变薄;病理切片HE染色,镜下可见心肌纤维断裂溶解,心肌细胞坏死,大量炎性细胞浸润。结论:在小鼠心肌梗死模型制备中,采用改良气管插管法,操作简单、易行、创伤小,模型制备成功率高。
Objective: An improved tracheal intubation method was used in preparation of myocardial infarction model in the mouse for increasing the success rate. Methods: The mice were intubated by an improved trachea cannula through oral cavity. The left anterior thoraco- tomy performed. The myocardial infarction model was made by legating the left anterior descending coronary artery in mouse. The color of heart was observed, and electrocardiogram was recorded. The survival rate and pathologic change were observed after two weeks of operation. Resuits: 40 myocardial infarction model mice were made by improved trachea cannula. The color of ventricles anterior wall had got madder red, and ST stages were raised onⅡ leads of electrocardiogram in all of model mice. After two weeks, 27 mice were survival. The survival rate was 87.1% except for accidental death during operation. The heart chamber expanded and ventricular wall became thin in myocardial infarction mice by eyes. After pathological sections were stained, by HE cardiac muscle fibers ruptured or lysed. There were some of necrosis of myocardiac cells and many of infiltration of inflammatory cells. Conclusion: Application of an improved tracheal intubation method simplified operation of tracheal intubation during preparation of myocardial infarction model in the mouse. The trauma was tinier than the other one, and achievement ratio of the model preparation was improved.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2011年第3期380-383,388,共5页
Chinese Journal of Applied Physiology
基金
遵义市红花岗区科技局基金(遵红科合社字(2007)10号)
关键词
小鼠
气管插管
心肌梗死
mouse
tracheal intubation
myocardial infarction