尖孢镰刀菌亚麻专化型生物学特性研究

Study on biological characteristics of Fusarium oxysporum Schl.f.sp.lini

文章旨在研究尖孢镰刀菌亚麻专化型病原体的生物学特性。探索温度、pH、光照、培养基、碳源及氮源等条件对该病原体菌丝生长、分生孢子产生和萌发的影响。结果表明,该菌菌丝分别在30℃、pH 7、8条件下生长最快;持续黑暗及PDA培养基上生长状况最好;可有效利用多种碳、氮源,其中以淀粉、氯化铵最适。分生孢子的产量及萌发率分别在25、30℃,pH 7、8条件下最高;光照亦利于孢子的产生和萌发;PSA培养基适于产孢,PDA培养基适于孢子萌发;所试条件的产孢及孢子萌发最适碳源分别为乳糖、果糖,氮源为氯化铵及L-半胱氨酸。系统掌握了该病原体的生物学特性,为亚麻田间病害防治提供了理论依据。

This paper aimed to study the biological characteristics of the Fusarium oxysporum Schl.f.sp.lini.The effects of temperature,pH,illumination,medium and the sources of nitrogen and carbon on the mycelium growth,the emergence and germination of conidiospore of the pathogen were explored;The results showed that for the mycelium growth,the optimum were 30 ℃,pH 7,8.Continuous darkness and PDA medium were favorable for growth of the pathogen.It could also effectively use all kinds of carbon and nitrogen sources,and the optimal carbon source was soluble starch,nitrogen source was ammonium chloride.The production and germination rate of conidiospore reached at the highest in 25,30 ℃ and pH 7,8,respectively.Light was favorable for the emergence and germination of conidiospore.The most suitable medium for emergence of conidiospore was PSA,and for the germination was PDA.The optimum carbon source was lactose and fructose,nitrogen source was ammonium chloride and L-Cystine,respectively for the spore emergence and germination;The study of this paper systematically made a research on the biological characteristics of Fusarium oxysporum Schl.f.sp.lini.It provided a theoretical basis for the prevention and control of the flax disease in the field.