SYBR Green I荧光检测痕量宿主DNA

Detecting trace host DNA based on SYBR Green I fluorescence method

基于SYBR Green I荧光染料与双链DNA结合产生荧光的原理,建立一种痕量DNA荧光检测方法。采用不同稀释倍数的SYBR Green I荧光染料做DNA的标准曲线,确定SYBR Green I荧光染料的最佳稀释倍数。比较荧光法和Southern blotting杂交法定量DNA。结果表明,SYBR Green I荧光染料稀释倍数为1∶10 000时线性范围广,线性关系好(R2=0.9999);荧光法和Southern blotting杂交法可以相互印证。由此可知,SYBR Green I荧光法定量DNA是一种快速、稳定的痕量DNA检测方法。

A fluorescence method for trace amount DNA detection was established based on the principle that combination of SYBR Green I fluorescent dyes and double-stranded DNA will generate fluorescent.Using different dilution of SYBR Green I fluorescent dye to do DNA standard curve to confirm the optimization of dilution for the SYBR Green I fluorescence dye.Comparison of detection results of quantitative DNA analysis between fluorescence and Southern blotting hybridization.The results showed that it could obtain the wide linear range and better linear relationship（R2=0.9999） when the dilution of SYBR Green I fluorescent dye was 1∶10 000,and the methods of fluorescence hybridization and Southern blotting could permit each other.SYBR Green I fluorescent analysis of quantitative DNA is a fast and stable detection method of trace DNA.