利用α-淀粉酶和β-淀粉酶制备缓慢消化淀粉

Preparation of slowly digestible starch by α-amylase and β-amylase

以普通玉米淀粉为原料,分别利用α-淀粉酶和β-淀粉酶制备缓慢消化淀粉(SDS),并通过正交试验优化了制备SDS的最佳工艺条件。通过正交试验确定α-淀粉酶法制备SDS的最佳条件为:pH 6.5,酶反应时间50min,温度50℃,α-淀粉酶用量240 U,在此条件下SDS最高产率为7.11%;通过正交试验确定β-淀粉酶法制备SDS的最佳条件为:pH 7.0,酶反应时间60 min,温度40℃,β-淀粉酶用量500 U,在此条件下SDS最高产率为10.12%。由于两种制备方法的最佳工艺条件差别不大,而β-淀粉酶法制备的产品产率较高。

Making normal corn starch as original material,SDS was prepared by α-amylase and β-amylase respectively.The orthogonal test optimal conditions were given as follows,α-amylase： pH 6.5,enzyme reaction time of 50 min,temperature 50 ℃,α-amylase 240 U;β-amylase： pH 7.0,enzyme reaction time of 60 min,temperature 40 ℃,β-amylase 500 U.Based on these conditions,starch products prepared using α-amylase and β-amylase showed yield of SDS by 7.11% and 10.12%.Although the optimum conditions of the two methods are similar,the products prepared using β-amylase with higher yield.