温度和光照对微藻的生长、总脂肪含量及脂肪酸组成的影响

EFFECT OF TEMPERATURE AND ILLUMINATION ON THE MICROALGAE'S GROWTH, TOTAL LIPID AND FATTY ACID COMPOSITION

用温度梯度法、二因子重复试验法分别研究温度、光照周期和光照强度对绿色巴夫藻的生长、总脂肪含量以及脂肪酸组成的影响，用正交试验法研究以上三因子对等鞭藻3011的影响。结果表明，温度20℃、光照强度120．19μmol／（m2·s）、光照周期L：D＝16：8最适于等鞭藻3011的生长及脂肪和PUFA的合成。绿色巴夫藻适宜在15～30℃内生长，但在20℃、9615μmol／（m2·S）及L：D＝18:6的条件下，能促使它较快生长且能合成较多的脂肪和必需脂肪酸。两种微藻ω3／ω6显著性地受到环境因子的综合影响。

The influence of temperature, light period and light intensity on growth, total lipid and fatty acid composition of Pavlova viridis was conducted with temperature gradient, repeated experiment of other two factors respectively, and that of Isochrysis galbana 3011 was conducted with orthogonal experiment of above three factors. The results showed that temperature 20℃, light intensity 120. 19μmol/(m2 ·s) and light period L:D= 16: 8 was the optimum culture condition for I. galbana 3011. The P. viridis were suitable to grow at 15~ 30℃. But it could grow faster and produce more lipid and EFA at 20℃, 96. 15μmol/(m2·s ), L: D= 18: 6. The ω3/ω6 of the two microalgae was synthetically affected by environmental factors.