头穴透刺对急性脑出血家兔脑组织N-乙酰基天门冬氨酸和胆碱化合物水平的影响

Effect of Penetrative Needling of Scalp-acupoints on Cerebral N-acetyl-aspartate and Choline Levels in Intracerebral Hemorrhage Rabbits

目的:观察头穴透刺对脑出血家兔脑内N-乙酰基天门冬氨酸(N-acetyl-aspartate,NAA)和胆碱化合物(choline,Cho)的影响,探讨头穴透刺法对脑出血后神经损伤的作用机制。方法:30只新西兰兔随机分为正常对照组、模型对照组和针刺组,每组10只。采用自体血二次注血法复制急性脑出血模型。针刺组造模成功后选取患侧"百会"透"太阳"穴治疗,留针30 min,留针期间施以平补平泻法捻转,每日1次,连续治疗7 d。分别在造模前及造模后2 h、3 d和7 d对各组家兔进行磁共振波谱分析NAA、Cho及肌酸(Cr)各峰下面积,观察血肿周围NAA/Cr、Cho/Cr比值的变化。结果:与正常对照组相比,兔脑出血模型建立后血肿周围NAA/Cr比值显著下降(P<0.05),Cho/Cr比值显著升高(P<0.05)。针刺治疗后NAA/Cr水平明显高于模型对照组(P<0.05),Cho/Cr水平明显降低(P<0.05)。结论:透刺"百会""太阳"穴可调节急性脑出血家兔脑组织内NAA/Cr和Cho/Cr代射变化,从而加快了脑组织神经损伤的修复过程。

Objective To observe the effect of scalp-acupuncture on N-acetyl-aspartate（NAA） and choline （Cho） levels in the brain tissue in rabbits with intracerebral hemorrhage（ICH） so as to explore its underlying mechanisms in resisting neural lesion, Methods A total of 30 Newzealand rabbits were randomly divided into normal, model and acupuncture groups （n = 10）. ICH model was duplicated by injection of the rabbit-own blood （200 μL） into the bilateral Basal Nuclei （A： 2 mm; R, L： 5 mm; H： 10 mm）. A filiform needle was inserted into ＂Baihui＂ （GV 20） to ＂Taiyang＂ （EX-HN 5） on the hemorrhage side, manipulated for 1 min, and then retained for 30 min （manipulated once again every 10 rain）. The treatment was conducted once daily for 7 days. Contents of NAA, Oho and creatine （Or） in the intracerebral peri-hematoma tissues were detected by using ^1H-Magnetic Resonance Spectroscopy （MRS）. Results Compared with the normal group, NAA/Cr ratios at the time points of 2 h, 3 d and 7 d after IGH in the model group were decreased markedly （ P〈0.05）, while Oho/Gr ratios at the same 3 time-points after ICH in the model group were increased markedly （P〈0.05）. In comparison with the model group, NAA/Cr ratio on day 3 after IOH in the acupuncture group was obviously higher （ P〈0.05）, while Cho/Or ratios on day 3 and 7 were significantly down-regulated in the acupuncture group （ P〈0.05）. No significant changes were found in NAA/Cr ratios at 2 h and on day 7 and in Oho/Or ratio at 2 h after ICH between the acupuncture group and the model group. Conclusion Penetrative acupuncture of GV 20-EX-HN 5 is effective in suppressing ICH-induced increase of Cho/Cr ratio in ICH rabbits, which may contribute to its function in accelerating neural repair process.