针刺“足三里”穴对脾虚证大鼠血清中睾酮和雌二醇水平的影响

Effect of Acupuncture of "Zusanli" (ST 36) on Sexual Hormone Levels in Spleen-deficiency Syndrome Rats

目的:观察针刺"足三里"对脾虚证大鼠内分泌代谢变化的影响,探讨其作用机制。方法:选用成年雄性Wistar大鼠26只,随机分为正常对照组(n=6),脾虚造模组(n=8),自然恢复组(n=6),针刺治疗组(n=6)。除正常对照组外,其余3组均用酒精+白醋灌胃(1 mL/100 g),建立脾虚证模型。针刺治疗组给予针刺"足三里"治疗,每天1次,共治疗10 d。用放射免疫方法测定血清中睾酮(T)、雌二醇(E2)的含量。结果:与正常对照组比较,脾虚造模组T、E2含量明显降低,自然恢复组与脾虚造模组比较血清中T、E2含量没有明显变化(均P>0.05),针刺"足三里"穴可使脾虚大鼠血清中T、E2含量及T/E2比值升高,明显高于脾虚造模组(P<0.05)。结论:针刺"足三里"穴治疗脾虚大鼠后,对内分泌代谢具有调节作用,性激素接近正常水平,使大鼠体质得以恢复。

Objective To study the underlying mechanism of acupuncture of ＂Zusanli＂ （ST 36） in ctinical treatment of spleen deficiency syndrome （SDS）. Methods Twenty-six adult male Wistar rats were randomized into normal control group （n = 6）, model group （n = 8）, saline ＋ model （saline） group （n = 6） and acupuncture ＋ model （acupuncture） group （n = 6）. The spleen deficiency syndrome model was duplicated by intragastric perfusion of 50% alcohol （1 mL/100 g, once） and vinegar （pH 3, 1 mL/100 g, once daily for 10 d）. Bilateral ＂Zusanli＂ （ST 36） was punctured with filiform needles which were manipulated for 1 min after insertion and retained for 20 min. The treatment was conducted once daily for 10 days. The contents of serum testosterone（T） and estradiol （E2）were detected by radioimmunoassay. The rats＇ physical strength was detected by exhausted swimming test in cool water. Results In comparison with the normal group, the duration of exhausted swimming, serum T and E2 contents and T/E2 were decreased significantly in the model group （P〈0.05）. Compared with the model group, the duration of exhausted swimming, serum T and E2 contents and T/E2 in the acupuncture group were upregulated significantly （P〈0.05）. No significant differences were found between the saline and control groups （P〉0.05）. Conclusion Acupuncture of ＂Zusanli＂ （ST 36） is able to reverse SDS-induced decrease of sexual hormones in spleen deficiency syndrome rats, which may contribute to its effect in improving physical strength.