茄子子叶下胚轴离体再生体系建立

Establishment of Regeneration System of Cotyledon and Hypocotyl in vitro of Eggplant

以3份茄子材料无菌苗子叶和下胚轴为外植体,研究其在MS附加不同浓度6-BA和NAA的培养基上的分化情况。结果表明:在不同培养基上,茄子子叶和下胚轴均能诱导出愈伤组织和分化形成不定芽,但不定芽诱导率存在明显差别,诱导3份材料下胚轴愈伤组织形成和芽分化的最佳培养基均为:MS+6-BA 2.0mg/L+NAA 0.1mg/L,子叶因品种不同有较大差异,紫色长棒茄芽分化最佳生长调节物质组合是6-BA 1.0mg/L+NAA 0.5mg/L;紫红线茄是6-BA2.0mg/L+NAA 0.1mg/L;紫黑短棒茄是6-BA 3.0mg/L+NAA 0.5mg/L。

The cotyledon and hypocotyl from three eggplant asepsis seedlings were cultured on the medium with different 6-BA and NAA concentrations to study their effects on the callus induction and shoot differentiation.The results indicated that some callus could be induced and adventitious buds could be differentiated in all kinds of medium,but there were obvious different in different medium.The optimum medium for eggplant callus formation and bud differentiation from hypocotyls was MS＋6-BA 2.0 mg/L ＋NAA 0.2 mg/L and the best medium for bud differentiation from cotyledon was different between different samples,there were MS＋6-BA 1.0 mg/L ＋NAA 0.5 mg/L for sample 1（puple,long clavate eggplant）,MS＋6-BA 2.0 mg/L ＋NAA 0.1 mg/L for sample 2（mauve,line shape eggplant）and MS＋6-BA 3.0 mg/L ＋NAA 0.5 mg/L for sample3（modena,short clavate eggplant）respective.