“爱神玫瑰”葡萄杂交胚胚性愈伤组织诱导与保持的研究

Studies on the Initiation,Maintenance of Embryogenic Callus of Hybird Embryo 'Aishenmeigui'

以无核葡萄"爱神玫瑰"的杂交胚为试材,通过试验筛选出了胚性愈伤诱导基本培养基:NN69;胚性愈伤组织诱导培养基:NN69+TDZ 0.05mg/L+2,4-D 0.5mg/L;胚性愈伤组织长期保持与增殖培养基:NN69+6-BA 0.2mg/L+2,4-D 0.1mg/L和NN69+2,4-D 0.2mg/L+TDZ 0.1mg/L为"爱神玫瑰"较适宜的培养基。

This study was carried on immature zygotic embryos of seedless grapes ＇Aishenmeigui＇about callus inducton and embryogenic callus maintenance.For ＇Aishenmeigui＇ callus initiation media was solid NN69 media combinationed of 2,4-D 0.5 mg/L and TDZ 0.05 mg/L.Embryogenic callus maintaim and proliferation media was NN69 supplemented with 6-BA 0.2 mg/L＋2,4-D 0.1 mg/Land NN69 supplemented with 2,4-D 0.2 mg/L＋TDZ 0.1 mg/L.