摘要
以丝瓜基因组DNA为模板,采用正交实验设计L16(45),在4个水平上对影响丝瓜SRAP反应的Taq酶、Mg2+、模板、dNTP及引物等5个因素进行了优化,建立了丝瓜SRAP-PCR的最佳反应体系。利用18份丝瓜自交系材料来验证此反应体系,均可扩增出清晰、可辨的条带,可见该反应体系较稳定,适用于丝瓜SRAP标记的扩增。
Loofah genom DNA was used for the PCR template to optimize loofah SRAP-PCR reaction system.Orthogonal experiments were designed to optimize the key parameters including Taq polymerase,Mg2＋,template DNA,dNTPs,primer and so on.A SRAP-PCR system with good stability was established.The optimized SRAP-PCR system was tested by 18 loofah inbred lines and was steady and reliable.
出处
《北方园艺》
CAS
北大核心
2011年第15期160-162,共3页
Northern Horticulture
基金
广州市科技局资助项目(2010Z1-E381)
关键词
丝瓜
SRAP
体系优化
loofah
SRAP-PCR
system optimization
