苦瓜子叶离体再生体系的建立

Establishment of Regeneration System in vitro for Cotyledon of Momordica charantia L.

以"圣香"苦瓜、"玉华"苦瓜的子叶为外植体进行离体培养植株再生研究。结果表明:2个苦瓜品种的子叶在不同的培养基上都较易形成愈伤组织,愈伤组织诱导率都达到80%以上。培养基MS+TDZ 0.05mg/L+NAA 0.02mg/L适合"圣香"苦瓜子叶不定芽分化,分化率为68.4%;培养基MS+TDZ 0.03mg/L+NAA 0.02mg/L适合"玉华"苦瓜子叶不定芽分化,分化率为67.8%。"圣香"苦瓜在培养基上MS+ZT 0.3mg/L+NAA 0.01mg/L上丛生芽诱导效果好,增殖率达6.5;"玉华"苦瓜丛生芽诱导的最佳培养基为MS+TDZ 0.02mg/L+NAA0.01mg/L,增殖率达6.6。生根诱导以1/2MS+NAA 0.05mg/L培养基诱导率最高,"圣香"苦瓜生根率为90.7%,"玉华"苦瓜生根率达91.9%。

Using cotyledon as explants,tissue culture and plant regeneration of Momordica charantia L.had been studied.The results showed that explants of Momordica charantia L.were easily induced to grow callus.The rate of callus formation were above 80%.MS medium supplenmented with TDZ 0.05 mg/L＋NAA 0.02 mg/L was best for bud induction of ＇Shengxiang＇ in which the induction rate was 68.4%.The best bud regeneration medium of ＇Yuhua＇ was MS＋TDZ 0.03 mg/L＋NAA 0.02 mg/L in which the induction rate was 67.8%.The most appropriate medium for multiplication of cluster buds of ＇Shengxiang＇ was MS＋ZT 0.3 mg/L＋NAA 0.01 mg/L.The coefficient of multiplication 6.5.MS medium containing TDZ 0.02 mg/L＋NAA 0.01 mg/L gave best result for cluster buds of ＇Yuhua＇ in which the rate of multiplication was 6.6.1/2MS medium containing NAA 0.05 mg/L was best for root induction and the rate of rooting for ＇Shengxiang＇ and ＇Yuhua＇ was 90.7% and 91.9%,respectively.