摘要
针对葡萄组织中多酚、多糖类物质含量较高的特点,对比研究了利用植物总DNA提取试剂盒和改良CTAB法提取葡萄总DNA。结果表明:改良的CTAB法能够从不同葡萄品种和不同生长时期的叶片中获得高质量、完整性好的总DNA,其OD260/OD280介于1.7～1.9之间,无降解现象,RNA去除干净,完全适于进行PCR和酶切等研究。
Extracting high-quality DNA from grapevine tissues were particularly difficult due to high levels of polyphenolics and polysaccharides,commercial total DNA extraction kit and a improved CTAB method were applied to isolate DNA.The results showed that the improved CTAB method isolate high purity and integrity DNA from different varieties and different growing stages of the leaves.The ratio of OD260/OD280=1.7~1.9.Non-degradation phenomenon took place,and RNA removed clearly.The DNA was suitable for PCR and enzyme digestion.
出处
《北方园艺》
CAS
北大核心
2011年第15期172-174,共3页
Northern Horticulture
基金
宁夏回族自治区科技攻关项目国际科技合作专项资助项目
宁夏自然科学基金资助项目(NZ09193)
关键词
葡萄
DNA提取
PCR
酶切
grapevine
isolating DNA
PCR
enzyme digestion
