红肉苹果组织培养及转基因体系的建立与优化

Establishment and Optimization of Tissue Culture and Genetic Transformation System of Malus sieversii var. niedzwetzkyana

于早春取红肉苹果植株上当年生茎段,建立其组织培养体系,并对其组织培养体系进行优化。结果表明:在添加0.2mg/L IBA+1.0mg/L TDZ的MS培养基上红肉苹果叶盘不定芽诱导率最高,再生率为100%,平均每个叶盘再生9.57个不定芽;1mg/L IBA+1mg/L BAP组合有利于植株扩繁,其扩繁率为8.27;添加0.6mg/L IBA的培养基上的生根率最高,为93.3%。转基因测试结果表明,农杆菌菌株EHA105适于红肉苹果的基因转化;农杆菌浸染后进行3d的共培养有利于转化效率的提高。

Shoots of Malus sieversii var.niedzwetzkyana were adopted in early spring,the tissue culture system was established and optimized using of these plant materials.The influence of different phytohormone combination on shoots regeneration,propagation and root formation was studied.The results indicated that a combination of 0.2 mg/L IBA＋1 mg/L TDZ was most suitable for adventitious shoot regeneration from leaf explants（100% regeneration rate and 9.57 adventitious shoots per explants）;the combination of 1 mg/L IBA＋1 mg/L BAP was optimum for shoot propagation with the propagation rate of 8.27 root formation rate（93.3%） on medium supplemented with 0.6 mg/L IBA was the highest.The results of transformation experiments showed that Agrobacterium strains EHA105 was suitable for the genetic transformation and 3 days of pre-culture support strongly the transformation efficiency.