叶用芥菜游离小孢子的培养及其植株再生

Microspore Culture and Plantlet Regeneration in Leaf Mustard Brassica juncea

为了利用小孢子培养以加速基因型的纯合,提高育种的选择效率,加速育种进程,以叶用芥菜6个基因型为材料,研究了游离小孢子培养诱导的胚状体和植株再生。结果表明,不同基因型的胚产量差异很大。在接种的6个基因型中,有3个诱导出胚,诱导率为50%。其中,黔青2号×紫红青菜的胚产量最高,平均每个花蕾产生2.83个胚状体,最低的是黔青2号×普安青菜,平均每个花蕾产生0.21个胚状体。3个出胚的基因型都获得了再生植株。

The microspores from six leaf mustard genotypes were cultured to induce embryoids and plantlet generation for speeding up homozygous genotype, improving selection efficiency and accelerating breeding process. The results showed that there was significant difference in induced embryoid amount among six different genotypes, the embryoids could be induced from microspores of three genotypes and the induced rate was 50%, 2.83 embryoids （maximum） and 0.21 embryoid （minimum） were induced from qianqing 2 × Zihongqingcai and Qianqing 2 × Pu ＇ anqingcai respectively, and the plantlets could be generated from the embryoids induced from microspores of three genotypes.