贵州樱桃种质资源遗传多样性的分子评价

Molecular Evaluation of Genetic Diversity of Cherry Germplasm Resource from Guizhou Province

为贵州樱桃种质资源的进一步开发利用提供参考,采用RAPD标记,对35份贵州地方樱桃（Prunus pseudocerasu L.）种质资源的遗传多样性进行了分子评价。结果表明,从94条RAPD引物中筛选出30条稳定性强、谱带清晰及多态性丰富的引物进行PCR扩增,共获得298个标记,其中多态性标记为255个,多态性比例为85.57%,相似系数为0.44~0.87。表明,贵州地方樱桃资源的遗传多样性丰富;毕节、贵阳及黔南等地的樱桃资源具有丰富的遗传多样性,尤其毕节地区拥有大量品质优良的资源,可作为品质育种的亲本。

The genetic diversity of 35 cherry（Prunus pseudocerasu） accessions from Guizhou province was investigated using RAPD markers to provide a reference for further development and utilization of cherry germplasm resource in Guizhou province.The results showed that a total of 298 markers were scored from the 30 primers,which were screened out from 94 primers and demonstrated highly reproducible,clear and polymorphic bands.Among the obtained markers,255 were polymorphic,accounting for 85.57% of the total.The genetic similarity among the accessions ranged from 0.44 to 0.87,reflecting the high genetic diversity of cherry germplasms in Guizhou province.The UPGMA dendrogram demonstrated that the accessions from Bijie,Guiyang and Qiannan areas were more genetically diversified.In particular,the accessions from Bijie exhibited the highest degree of diversity in fruit quality,which may be used as elite materials for further genetic improvement in this species.