摘要
目的建立高效液相色谱法测定血浆中氨甲喋呤浓度的方法。方法以乙腈∶0.02mol/L磷酸水溶液(10∶90)为流动相,监测波长为302nm。结果在0～35.2μmol/L范围内呈良好线性关系,r=0.99995。日内及日间测得的RSD分别为0.62%,3.03%,平均回收率为99.6%。血清中最低检测浓度为10ng/ml。结论本法具有快速、准确,操作简便,流动相成本低等优点。
Objective To establish a method for the Determination of MTX in human serum by HPLC.Methods The mobile phase consisted of acetonitrile：0.02mol/Lphosphoric acid aqueos solution（10∶90）.UV Detector was set at 302 nm.Results The good linearity was from 0～35.2 （μmol/L）,r=0.99995.Within day and between days were 0.62% and 3.03% respectly.The average recovery was 99.6%.The detection of serum concentration was of 10ng/ml.Conclusion This method has advantages of fast,accurate,simple,low cost mobile phase.
出处
《临床合理用药杂志》
2011年第16期9-10,共2页
Chinese Journal of Clinical Rational Drug Use
关键词
高效液相色谱法
氨甲喋呤
血药浓度
High-performamce Liguid Chromatography
Methotrexate
Plasma concentration
