姜黄素单体影响肝癌BEL-7402细胞凋亡及其周期蛋白D表达的研究

Effects of curcumins on cyclin D expression and apoptosis of human hepatoma BEL-7402 cells

目的采用细胞学实验观察3种姜黄素单体对肝癌细胞株BEL-7402的抑制增殖及诱导细胞凋亡的作用强度和机制。方法以四甲基偶氮唑蓝(MTT)、AnnexinⅤ-FITC双标记及流式细胞术(FCM)实验观察3种姜黄素对肝癌细胞株BEL-7402增殖的抑制作用与细胞周期变化,采用Western blot实验分析3种姜黄素对肝癌细胞株BEL-7402细胞周期蛋白D表达的影响。结果 (1)3种姜黄素均可通过诱导肝癌细胞凋亡而有效抑制肝癌细胞生长增殖,存在时间与剂量效应,以姜黄素Ⅲ抑制效果最强。(2)3种姜黄素通过影响细胞周期生长的调控信号、降低细胞周期蛋白D表达量致使肝癌细胞停滞于G1/S期。结论 3种姜黄素均可通过抑制肝癌细胞周期蛋白D表达而诱导细胞凋亡,有效地抑制肝癌细胞生长增殖,具有很高的临床治疗价值。

Objective To study the effects of three kinds of curcumins on proliferation inhibition and apoptosis induction of the cell line of human hepatoma BEL-7402 and to elucidate their possible molecular mechanism.Methods The inhibiting ratio of 3 curcumins in different concentrations were determined by MTT assay at different times.Apoptosis was evaluated by agarose gel electrophoresis and Annexin V-FITC double pigmentation method.The cell cycle was detected by FCM.Western blot method were used to analysis the expression of cyclin D protein.Results（1）Three curcumins could obviously suppress BEL-7402 cell proliferation in time-and does-dependent manner,and curcumin III was the best.（2）Three curcumins induced BEL-7402 cancer cell to apoptosis by decreasing cyclin D expression and stopping the cancer cells in G1/S period.Conclusion Curcumins suppress the cancer cell BEL-7402 proliferation and induce its apoptosis through changing cyclin D expression,showing great values in human hepatom therapy.