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高效液相色谱法测定中药退黄外洗液中大黄酸、大黄素、大黄酚的含量 被引量:3

Determination of rhein,emodin and chrysophanol in Chinese Medicine Tuihuang outside lotion by HPLC
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摘要 目的建立中药退黄外洗液中大黄酸、大黄素、大黄酚的含量测定方法。方法采用高效液相色谱(HPLC)法测定大黄酸、大黄素、大黄酚的含量,色谱柱:Phenomenex Gemini5μm C18110A4.6×250.0mm5micron;流动相:甲醇-0.1%磷酸溶液(85∶15),流速:1.0ml/min,检测波长:254nm,柱温:35℃。结果大黄酸在1.6576~33.1520μg/ml、大黄素在1.4976~29.9520μg/ml、大黄酚在1.7040~34.0800μg/ml范围内线性关系良好(r=1)。大黄酸平均回收率为99.07%,RSD为0.85%(n=5);大黄素为99.14%,RSD为1.08%(n=5);大黄酚为99.94%,RSD为0.77%(n=5);阴性对照无干扰。结论该方法操作简便、稳定、专属性强,可作为该制剂的质量控制方法。 Objective To establish a method for determination of rhein,emodin and chrysophanol in Chinese Medicine Tuihuang outside lotion.Methods Determinedf rhein,emodin,chrysophanol by HPLC,column:Phenomenex Gemini 5μm C18 110A 4.6×250.0mm 5micron;Mobile phase:Methanol-0.1% phosphoric acid(85:15),flow rate:1.0ml/min,detection wavelength:254nm,column temperature:35℃.Results Rhein at 1.6576~33.1520μg/ml,emodin at 1.4976~29.9520μg/ml,chrysophanol at 1.7040~34.0800μg/ml range,had a good linear relationship(r=1).The average recovery rhein was 99.07%,RSD=0.85%(n=5);Emodin was 99.14%,RSD was 1.08%(n=5);Chrysophanol was 99.94%,RSD was 0.77%(n=5);The negative sample without interference.Conclusion This method is simple,stable,specific,and can be used for the quality control methods.
作者 宾驰 颜栋林
出处 《临床合理用药杂志》 2011年第17期42-44,共3页 Chinese Journal of Clinical Rational Drug Use
关键词 高效液相色谱法 中药退黄外洗液 大黄酸 大黄素 大黄酚 HPLC Chinese Medicine Tuihuang outside lotion Rhein Emodin Chrysophanol
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