d-δ-三烯生育酚对人鼻咽癌CNE2细胞株增殖和凋亡的影响

Effect of d-δ-tocotrienols on the proliferation and apoptosis of nasopharyngeal carcinoma CNE2 cell line

目的探讨d-δ-三烯生育酚对人鼻咽癌细胞增殖与凋亡的影响。方法实验设对照组和不同d-δ-三烯生育酚浓度(40、50、60μmol/L)处理的实验组,采用Hoechst荧光染料染色和显微镜观察细胞的形态。3-(4,5二甲基噻唑-2)-2,5-二苯基四氮唑溴蓝(MTT)实验、流式细胞术分析d-δ-三烯生育酚对CNE2细胞的增殖、周期和凋亡的影响。结果 d-δ-三烯生育酚处理CNE2细胞48 h后,随d-δ-三烯生育酚的浓度升高,细胞生长抑制率明显增加,分别为(27.71±1.01)%、(57.09±0.56)%和(95.04±0.17)%,与对照组相比,差异具有统计学意义(P<0.01);细胞核或细胞浆内出现致密浓染的颗粒状或条块状荧光的凋亡细胞明显增多,实验组细胞凋亡率分别为(9.18±1.86)%、(34.97±1.01)%和(52.97±2.77)%,与对照组(1.58±0.48)%比较,随浓度升高,凋亡细胞率增加,差异具有统计学意义(P<0.01);实验组S期的细胞百分率分别为(25.40±1.93)%、(35.83±3.74)%和(47.13±2.49)%,与对照组(19.63±1.46)%比较,细胞呈S期阻滞,差异具有统计学意义(P<0.05)。结论 d-δ-三烯生育酚具有抗鼻咽癌CNE2细胞的生物学活性。

Objective To investigate effect of d-δ-tocotrienols on proliferation and apoptosis of nasopharyngeal carcinoma. Meth- ods The experiment was carried out with control group and experimental group which included three different concentration of d-δ-tocotrienols（40,50,60 μmol/L）. Cytomorphology was analyzed by using Hoechst 33 258 fluorescent staining and microscopy. MTT experiment and flow cytometry were used to analyze the effect of d-δ-tocotrienols on proliferation,apoptosis and cell cycle of nasopharyngeal carcinoma cell line CNE2. Results After treated CNE2 cells with d-δ-tocotrienols for 48h,cell growth suppression rate rose obviously with the increase of d-δ-tocotrienols concentration, respectively be （27.71 ± 1.01）%, （ 57. 09 ± 0. 56） % and （95.04± 0.17）%, the differences were Significant when compared with the control group （P〈0.01）. Apoptotic cells with nucleus and cytoplasma compact dyeing or thick strip massive fluorescence increased obviously, the apoptotic cell rates were （9. 18 ± 1.86） %, （34.97 ± 1.01） % and （52.97 ±2.77） % respectively in experimental group, compared with the control group （1.58 ± 0.48）%,cell apoptosis rate significantly increased, the differences were significantly （P〈0.01）. The percentage of S phase cells were （25.40 ± 1.93）%, （35.83 ± 3.74）% and （47.13 ± 2.49）% respectively in experimental group, cells were stopped at S phase, compared with control group （19.63± 1.46） % ,the differences were statistically significant （P〈0.01）. Conclusion d-δ-tocotrienols has biological activity of anti-nasopharyngeal carcinoma CNE2 cell line.