MMP-9血清水平及其基因-1562C/T多态性与儿童哮喘关系的研究

Study on association of serum matrix metalloproteinase-9 concentration and gene-1562C/T polymorphism to childhood asthma

目的探讨基质金属蛋白酶-9(MMP-9)血清水平及其基因-1562C/T多态性与儿童哮喘的相关关系。方法采用双抗体夹心酶联免疫吸附试验(SELISA)测定65例哮喘儿童(病例组)和68名健康对照儿童(对照组)血清MMP-9水平,并采用多聚酶链反应-限制性片段长度多态性(PCR-RFLP)分析技术检测MMP-9基因-1562C/T多态性。结果病例组儿童MMP-9血清水平(136.53±29.96)ng/mL明显高于对照组儿童[(45.08±12.53)ng/mL](P<0.05)。病例组和对照组MMP-9基因-1562的CC、CT、TT基因型构成比分别为67.7%、29.2%、3.1%和73.5%、25.0%、1.5%,其C和T基因频率分别为82.3%、17.7%和86.0%%、14.0%,两组的基因型和基因频率差异均无统计学意义(P>0.05)。病例组和对照组中不同基因型儿童的MMP-9血清水平差异均无统计学意义(P>0.05)。结论 MMP-9血清水平与儿童哮喘的发生、发展相关,而MMP-9基因-1562C/T的多态性与儿童哮喘发病无关。

Objective To explore the association of serum matrix metallproteinase 9 （MMP-9） concentration and gene -1562C/T polymorphism to childhood asthma. Methods The serum level of MMP-9 was determined by sandwich enzyme-linked immunosor bent assay （SELISA） method in 65 children with asthma （case group） and 68 healthy children （control group）,and the polymorphism of MMP-9 gene -1562C/T was detected by polymerase chain reaction and restriction fragment-length polymorphism （PCRRFLP） analysis technique. Results The serum level of MMP-9 in case group （136.53±29.96）ng/mL was higher than that in control group[（45.08±12.53）ng/mL,P〈0.05）1. The frequency of CC,CT,TT genotype in case group and in control group were 67.7%,29.2%,3.1% and 73.5%,25.0%,1.5%,and the gene frequency of C and T were 82.8%,17.7% and 86.0% ,14.0% respectively. There were no statistical significance in genotype and gene frequency between ease group and control group （P〉 0.05）. There were no statistical significance in serum level of MMP-9 of case group and control group among children with different genotypes （P〉0.05）. Conclusion The serum level of MMP 9 is closely associated to the occurrence and development of childhood asthma,but the polymorphism of MMP-9 gene 1562C/T have no correlation with the pathogenesis childhood asthma.