BMP13转染C_3H_(10)T_(1/2)细胞对改善心梗大鼠心功能的影响

BMP13 on C_3H_(10)T_(1/2) cells to improve cardiac function in myocardial infarction rats

目的:探讨在大鼠心肌梗死微环境中骨形态生成蛋白13(Bone morphogenetic protein,BMP13)能否促进C3H10T1/2细胞向心肌样细胞分化并且改善心梗大鼠的心功能。方法:56只SD(Sprague dawley)雄性大鼠随机分成4组:假手术组(SHAM,n=8只),单纯心梗组(Myocardial infarction,MI,n=16只),MI+pAdEasy-GFP重组腺病毒感染干细胞组(MI+GFP,n=16只),MI+pAdEasy-BMP13重组腺病毒感染干细胞组(MI+BMP13,n=16只)。各组体重检测,1次/周;超声心动图检测各组大鼠基线水平及术后4周心功能,比较各组指标变化及差异;Massons’染色比较各组心脏梗死面积差异;免疫荧光检测被植入各组细胞心肌特异性结构蛋白α-MHC及cTnT的表达。结果:重组腺病毒感染C3H10T1/2细胞24 h,每组约80%细胞有绿色荧光表达。术后4周超声心动图显示植入细胞组心功能改善,MI+BMP13组更为明显(P<0.05)。冰冻切片免疫荧光显示MI+GFP组和MI+BMP13组植入细胞处可见α-MHC及cTnT表达。结论:心梗微环境中BMP13可诱导C3H10T1/2细胞向心肌样细胞分化并改善心梗大鼠心功能。

Objective：To investigate whether the bone morphogenetic protein 13（BMP13） can promote C3H10T1/2 cell differentiation into cardiomyocytes in microenvironment and improve the cardiac function of the rats after myocardial infarct（MI）.Methods：MI and sham operation were established in sprague dawley（SD） male rats.Totally 56 rats were divided into 4 groups： sham operation group（8 rats）,MI group（16 rats）,MI＋GFP group（16 rats）,and MI＋BMP13 group（16 rats）.Left ventricular（LV） function were assessed by echocardiography on day baseline and 4 weeks after operation.After measuring body weight once a week,on 4 weeks after completion of cardiac function test,rats were sacrificed to take the hearts.Differences in infarct sizes of hearts were compared by Masson＇s staining.Cardiac-specific structural protein α-MHC and cTnT were implanted in each group and detected by immunofluorescence assay.Results：Each C3H10T1/2 cells had about 80% of cells expressing green fluorescence.On 4 weeks after the experiment,heart function in the groups with cells implanted significantly improved,as compared with MI group,MI＋BMP13 group more obvious（P〈0.05）.Implanted cells in group MI＋GFP and MI＋BMP13 had cardiac cell-specific structural protein α-MHC and cTnT expression by immunofluorescence.Conclusion：BMP13 can induce differentiation of C3H10T1/2 cells into myocardial cells and improve cardiac function in myocardial infarction rats.