雌激素对卵巢切除大鼠血脂和血清瘦素、抵抗素表达的影响

Effects of estrogen on blood lipids and the expression of serum leptin and resistin in ovariectomized female SD rats

目的:探讨雌激素对卵巢切除大鼠血脂和血清瘦素、抵抗素表达的影响。方法:12周SD大鼠分成3组,假手术(Sham)组、卵巢切除(OVX)组和雌激素(OVX+E2)组,每组7只,术后10 d,Sham组和OVX组大鼠每天腹腔注射生理盐水0.1 ml/只,OVX+E2组每天注射苯甲酸雌二醇0.2 mg/只,连续注射30 d后处死取血,全自动生化分析仪检测血脂,ELISA检测血清瘦素、抵抗素含量。结果:卵巢切除术后10 d,手术组体重增加明显,术后40 d,卵巢切除组大鼠总胆固醇和LDL均增加,OVX+E2组体重较OVX组下降,血清甘油三酯升高,LDL降低,总胆固醇含量降低。OVX组和OVX+E2组血清瘦素、抵抗素水平均无明显变化(P>0.05)。结论:卵巢切除可引起体重增加,脂质代谢紊乱,补充雌激素可降低体重,部分纠正血脂紊乱,卵巢切除和雌激素补充对血清瘦素和抵抗素水平影响不大。

Objective：To explore the effects of ovariectomy（OVX）and estrogen replace treatment on total cholesterol,LDL,HDL,triglycerides,serum leptin and resist levels.Methods：Female SD rats（12 weeks）were divided into three groups： control Sham,OVX and OVX treated with estrogen（OVX ＋E2）（n=7,each group）.10 days post operating,rats had been treated with estradiol benzoate（0.2 mg ip per day）（OVX ＋ E2）and vehicle（Sham and OVX rats）for 30 days respectively.Total cholesterol,LDL,HDL,triglycerides were analyzed by chemistry analyzer,and ELISA kits were employed to analyze serum leptin and resistin levels.Results：Body mass was increased by OVX and significantly decreased by estrogen treatment.Total cholesterol and LDL level were upregulated after OVX.Estrogen treatment increase triglyceride level,while decreased total cholesterol and LDL level of OVX rats.No affect was found in serum leptin and resistin level of OVX or estrogen treatment groups.Conclusion：OVX led to body mass increase and lipid metabolism disturbance.Treated with E2,the body mass of the ovariectomized rats were decreased and the disturbance of lipid metabolism was partially improved.OVX and estrogen replacement can not affect serum leptin and resistin levels.