创伤后应激障碍大鼠前额内侧皮质MR表达的变化

Change in expression of mineralocorticoid receptors in medial prefrontal cortex of rats with posttraumatic stress disorder

目的观察创伤后应激障碍(PTSD)样大鼠前额内侧皮质(medial prefrontal cortex,mPFC)神经元核受体-盐皮质激素受体(Mineralocorticoid receptors,MR)表达的变化。方法采用国际认定的单一连续应激(single prolonged stress,SPS)方法建立PTSD大鼠模型,取成年健康雄性Wistar大鼠90只,随机分为PTSD模型1d、7d、14d、28d和正常对照组。采用免疫组化、免疫印迹和RT-PCR方法分别进行各组mPFC神经元MR表达变化的观察及检测,进行图像分析和统计学处理。结果 PTSD大鼠mPFC神经元MR的表达在SPS-1d时高于对照组,随后下降,SPS-14d最低,SPS-28d恢复性上调,但仍然低于对照组(P<0.05)。结论 PTSD模型大鼠经SPS处理后,mPFC中出现MR表达的变化,该变化可能参与PTSD的下丘脑-垂体-肾上腺(hypothalamic pituitary adren axis,HPA)轴的变化机制。

Objective To observe the expression of mineralocorticoid receptors （MR） in the medial prefrontal cortex （mPFC） of rats with posttraumatic stress disorder （PTSD） . Methods The single prolonged stress （SPS） method was used to set up the rat PTSD models. A total of 90 male Wistar rats were randomly divided into ld, 7d, 14d, 28d of SPS groups and normal control group. Immunohistochemistry, Western blotting, RT-PCR, image analysis and statistical analysis were used to detect the activity of MR. Results The activity of MR in mPFC neurons increased at ld after exposure to SPS compared with that of the control group, then gradually decreased, reached the minimum at 14d, and restored to 28d, but still lower than that of the control group （P〈0.05）. Conclusion The expression of MR may be related to the pathoRenesis of HPA.