人外周血Th细胞和Tc细胞内IFN-γ及IL-4表达水平与白塞病的关系

Relatiaonship between expression level of IFN-γ and IL-4 expression in peripheral blood Th and Tc cells and Behcet's disease

目的检测BD患者外周血Th细胞和Tc细胞内INF-γ和IL-4的表达水平,探索BD患者体内的免疫状况。方法采用流式细胞术检测BD活动期患者(n=22),BD稳定期(n=6)以及健康体检者(n=22)外周血T细胞亚群INF-γ和IL-4的表达水平。结果 BD患者活动期外周血细胞Th细胞和Tc细胞经过刺激后胞内合成IFN-γ的水平明显高于正常人水平(Th细胞:22.45%±7.33%vs.16.32±4.96%,P<0.05;Tc细胞:36.74%±13.19%vs.28.67%±11.56%,P<0.05),稳定期患者Th细胞和Tc细胞体外合成IFN-γ的能力与正常人相比无明显差异;稳定期患者Th细胞和Tc细胞胞内合成IL-4的能力相对于正常人均无明显差异:(稳定期Th细胞:3.01%±1.38%vs.2.87%±1.46%,Tc细胞:1.14%±0.28%vs.1.07%±0.32%,P>0.05)。而活动期患者Th细胞和Tc细胞胞内合成IL-4的能力相对于正常人均存在明显差异:(Th细胞:3.54%±1.62%vs.2.87%±1.46%,Tc细胞:1.82%±0.43%vs.1.07%±0.32%,P<0.05);导致Th细胞和Tc细胞有偏向Th1及Tc1的趋势(Th细胞IFN-γ/IL-4:6.34±3.24 vs.5.69±4.72,p>0.05;Tc细胞IFN-γ/IL-4:20.19±13.65 vs.26.79±14.52,P>0.05)。结论 BD患者外周血INF-γ和IL-4的表达水平明显升高,并与疾病活动期存在一定程度相关,可能对白塞病患者的诊断具有重要的临床意义。

Objective To detect the percentages of intracytoplasmic IFN-γand IL-4 expression in peripheral blood Th and Tc cells from patients with BD,and to study the immunological condition of the BD patient.Methods Flow cytometry was used to detect the percentages of intracytoplasmic IFN-γand IL-4 expression in peripheral blood Th and Tc cells from patients with BD（active n=22,stable n=6） and normal individuals（n=22）,respectively.Results The percentages of intracytoplasmic IFN-γ expression in patients with BD（active） was higher than those of normal individuals（Th cell： 22.45%±7.33% vs.16.32±4.96 %,P0.05;Tc cell： 36.74%±13.19% vs.28.67%±11.56%,P0.05）,but they were insignificent between stable BD and normal controls.The percentages of intracytoplasmic IL-4 expression in patients with BD（active） was also higher（Th cell： 3.54%±1.62 % vs.2.87%±1.46 %,Tc cell： 1.82%±0.43% vs.1.07%±0.32%,P0.05）,but they were insignificent between stable BD and normal controls.（Th cell： 3.01%±1.38 % vs.2.87%±1.46 %,Tc cell： 1.14%±0.28% vs.1.07%±0.32%,P0.05）;so that Th and Tc cells deflect to Th1 and Tc1（Th cell IFN-γ/IL-4： 6.34±3.24 vs.5.69±4.72,p0.05;Tc cell IFN-γ/IL-4： 20.19±13.65 vs.26.79±14.52,p0.05）.Conclusion The percentages of intracytoplasmic IFN-γand IL-4 expression in peripheral blood Th and Tc cells from patients with active BD is significantly increased,which may be significant in the diagnosis of BD.