胃癌组织中幽门螺杆菌L型感染及骨桥蛋白检测的意义

Effect of helicobacter pylori L-form infection and expression of osteopontin on gastric carcinoma and its significance

目的通过检测胃癌组织中幽门螺杆菌L(HP-L)型感染以及OPN的表达情况,探讨Hp-L型感染对胃癌侵袭、转移生物学行为的影响及可能机制。方法①应用革兰染色及免疫组化(SP)法检测120例胃癌组织中的Hp-L型感染情况;②应用免疫组化(SP)法检测胃癌组织中的OPN的表达。分析Hp-L型感染与OPN表达的关系以及和临床病理因素之间的关系。结果①120例胃癌组织中有88例革兰染色检出Hp-L型,其阳性率为73.3%(88/120);免疫组化Hp-L型抗原表达阳性率为70.8%(85/120),革兰染色Hp-L型检出和Hp-L型抗原表达同时阳性的病例有82例(两者同时阳性者为Hp-L型阳性),其阳性率为68.3%(82/120)。②胃癌组织中Hp-L型阳性组的OPN表达阳性率分别为70.73%(58/82),高于Hp-L型阴性组表达率,且有统计学意义(P<0.005)。经四格表资料χ2检验,胃癌组中Hp-L型阳性与OPN阳性表达呈正相关(r=0.27,P<0.001)。③胃癌中Hp-L型阳性与肿瘤的大小、癌细胞的血管侵袭、浸润深度及淋巴结转移具有相关性(P<0.001-P<0.05)。结论 Hp-L型感染是引起胃癌侵袭、转移的重要因素之一,促进胃癌的发展,其机制可能与胃癌细胞的OPN表达量增加有关。

Objective To detect the helicobacter pylori L-form（Hp-L） infection and the expression of Osteopontin（OPN） in gastric carcinoma tissues,and to discuss the probable machanism between Hp-L infection and the progression of gastric carcinoma.Methods Gram＇s staining and immunohistochemical staining（SP method） were used to detect Hp-L in 120 gastric carcinoma cases.Immunohistochemical staining was also used to evaluate the expression of OPN.Results Hp-L was found in 88 and 85 cases of 120 gastric carcinoma tissues by Gram＇s and immunohistochemical staining,respectively.82（68.3%） cases were Hp-L positive by both Gram＇s and immunohistochemical staining.In the Hp-L positive group,58（58/82,70.73%）cases were OPN positive,while in the Hp-L negative group,16 cases were OPN positive,which had relationships to the size,invasion and metastasis of the tumor.Conclusion The infection of Hp-L plays an important role in the progression of gastric carcinoma.The increase in the intracellular levels of OPN may be one of the mechanisms.