慢性酒精中毒对成年小鼠小脑浦肯野细胞超微结构的影响

Effects of Chronic Ethanol Intoxication on the Ultrastructures of Cerebellar Purkinje Cells in Adult Mice

目的观察慢性酒精中毒所致的成年小鼠小脑皮质浦肯野细胞(Purkinje cell,PC)胞体的超微结构变化,探讨其对神经元超微结构的影响方式及生理意义。方法用15%酒精饲喂3月龄小白鼠3个月,经行为学检测后,取小脑前叶做电镜包埋,切片,染色,透射电镜下观察并拍照。结果酒精中毒组PC核周质中线粒体膨解,基质囊泡化;高尔基复合体扁平囊扩张;粗面内质网碎裂,核糖体颗粒减少;"空泡变性"出现;双层核膜界限不清;染色质边集等变化。结论慢性酒精中毒可导致小脑浦肯野细胞多种细胞器出现异常改变,推测这些变化可引起胞内物质合成减少,空间构筑紊乱,神经元死亡,最终导致小脑功能损伤。

Objective We observed chronic ethanol-induced ultrastructural alterations of Purkinje cell（PC） somata in the mouse cerebellar cortex,in order to explore the manner of ethanol impacts on neuronal ultrastructures and the physiological influences underlying these alterations.Methods 3-month old mice were fed with 15% alcohol for 3 months.After the behavioral test to manifest the symptoms of ethanol intoxication,the anterior lobe from each mouse cerebellum was selected for embedding,sectioning,and staining.Undera transmission electron microscope,the organelles of PC somata were observed and photos were taken.Results The organelles in ethanol-intoxicated PCs exhibited the following changes： the mitochondria swelled and the matrix decomposed;the sacs of Golgi apparatus dilated;the rough endoplasmic reticulum（rER） collapsed,accompanied with a great loss of the ribosomes;the ＂vacuolation＂ emerged;the double nuclear membrane became illegible;and the chromatin marginally condensed in the nucleus.Conclusion Chronic ethanol intoxication induces degenerative alterations in the organelles of cerebellar PCs,which might result in the decrease in substance synthesis,the disorder in intraneuronal configuration,the death of PCs.These alterations might ultimately lead to cerebellar impairments.