ADO在膀胱尿路上皮癌组织中的表达和意义

Expression and clinical significance of ADO in bladder urothelial cancer

目的探讨膀胱尿路上皮癌组织中腺苷(adenosine,ADO)的表达及临床意义。方法收集武汉大学人民医院病理科2000-2006年有完整临床和病理资料的膀胱尿路上皮癌存档蜡块50例和5例癌旁组织,采用免疫组织化学S-P法检测50例膀胱尿路上皮癌和5例癌旁组织中ADO的表达水平。采用HPIAS-1000高清晰度彩色病理图文报告管理系统,对ADO的表达进行定量分析,并用SPSS13.0软件对各组免疫组织化学反应阳性颗粒的平均光密度、阳性面积率做单因素方差分析和SNK(q)检验。结果 ADO在膀胱尿路上皮癌中呈低表达,癌旁组织中呈高表达。膀胱尿路上皮癌与癌旁组织相比,差异有显著性(P<0.05)。结论 ADO在膀胱尿路上皮癌中的低表达抑制了肿瘤细胞的凋亡,导致肿瘤细胞增生,ADO在膀胱尿路上皮癌的发生、发展中起了重要的促进作用。

Objective To investigate the expression and clinical significance of ADO in bladder urothelial cancer.Methods Parafflin-embeded specimen from 50 cases of bladder urothelial cancer and 5 cases of normal tissues near the bladder urothelial cancer,which were all filed with full pathological and clinical data,were collected from the pathology department of the Renmin Hospital of Wuhan University between 2000 and 2006.The expression of ADO was detected by immunohistochemical S-P method.Quantitative analysis of ADO was conducted by high definition pathological graphics context report system （HPIAS-1000）.One-way analysis of variance and SNK（q） tests were used to analyse the mean density and the positive area rate of the immunohistochemical results.Results The expression of ADO in bladder urothelial cancer was lower than that in normal tissues（P0.05）.Conclusion The low expression of ADO in bladder urothelial cancer suppresses the apoptosis of tumour cells and causes tumour cell proliferation,so ADO plays an important role in the occurrence and the development of bladder urothelial cancer.