细胞核内特征值的改变可引起DNA倍体的变化

Changes of nuclear features with different DNA ploid

目的通过测定不同DNA倍体细胞,研究细胞核内特征值的改变。方法用宫颈刷刷出宫颈细胞,经固定后,用涂片离心机制成二张玻片,一张行巴氏染色作TBS诊断,另一张行Feulgen染色做DNA定量测定。通过对宫颈细胞核图像内像素的统计,计算出细胞核内多种特征值,比较不同DNA倍体细胞内特征值的不同。结果 161873例妇女行宫颈细胞学检查,常规细胞学检查发现2454例低级别鳞状上皮内病变(low-grade squamous intraepithelial lesion,LSIL)和523例高级别鳞状上皮内病变(high-grade squamous intraepithelial lesion,HSIL);而DNA倍体分析发现3412例有3个以上>5c细胞。84%以上的LSIL和HSIL病例均可见倍体异常细胞。与2c细胞相比,4c、5c、7c及9c细胞核面积及核半径明显增大;7c、9c细胞核内平均光学密度和紧实度均值也有明显改变,而光密度方差和灰度熵无变化。结论宫颈细胞DNA倍体改变往往伴有细胞形态和DNA核内分布等特征值的改变。

Objective To study the nuclear features in different DNA ploids of cervical cells by DNA image cytometry.Methods Cervical cells were collected by a brush and fixed.Two slides per sample were made by a cytospin.One slide was stained by Pap solution for TBS diagnosis,whice the other one was stained by Feulgen for DNA ploidy analysis.Nuclear features,such as nuclear area and mean optical density（OD）,were also measured.Results Cervical samples from 161,873 women were examined.2454 low-grade squamous intraepithelial lesions（LSIL） and 523 high-grade squamous intraepithelial lesions（HSIL） were found by TBS diagnosis,while 3 and more than 3 5c aneuploid cells in 3412 cases were determined by a DNA image cytometer.Aneuploid cells were found in the samples of 84% LSIL and 96% HSIL cases.Compared to those of 2c cells,the nuclear area,radius,mean OD and compactness were significantly different in 7c and 9c cells,while variance and gray entropy were found the some change.Conclusion Nuclear features of cervical cells are changed with different DNA ploids.