17-丙烯胺基-17-去甲氧基格尔德霉素诱导鼻咽癌细胞凋亡的实验研究

Heat shock protein inhibitor 17-AAG induces apoptosis of HNE1 cell line in human nasopharyngeal carcinoma

目的:研究17-丙烯胺基-17-去甲氧基格尔德霉素(17-allylamino-17-demethoxygeldanamycin,17-AAG)对人鼻咽癌细胞株(Human nasopharyngeal carcinoma cell line,HNE1)增殖抑制和诱导凋亡的作用,探讨17-AAG在鼻咽癌临床治疗中的意义。方法:采用噻唑蓝(Thiazolyl blue,MTT)检测17-AAG对HNE1细胞的增殖抑制作用,流式细胞仪检测细胞凋亡率的变化,Hoechst33528染色观察细胞的凋亡形态学变化并进行核碎片计数。结果:17-AAG抑制HNE1的增殖,诱导了细胞的凋亡,抑制作用具有浓度和时间依赖性。结论:17-AAG可诱导HNE1的凋亡,研究结果对药物治疗鼻咽癌有一定的临床指导意义。

Objective：To observe the inhibitory effects of heat shock protein inhibitor 17-AAG on proliferation and induction of apoptosis of HNE1 cells in human nasopharyngeal carcinoma and investigate its clinical significance for the treatment of nasopharyngeal carcinoma.Methods：MTT reduction assay was used to measure cell viability.Flow cytometry was used to detect apoptotic cells.Hoechst33528 staining and fluorescence microscopy was used for morphological observation of apoptotic cells and calculation of nuclear fragmentations.Results：17-AAG inhibited proliferation and induce apoptosis of human nasopharyngeal carcinoma HNE1 cell line in vitro.The inhibition of proliferation was in time and dose dependent manner.Conclusion：17-AAG could induce human nasopharyngeal carcinoma cells to undergo apoptosis and can be a potential anti-cancer drug to treat nasopharyngeal carcinoma.