CDK13在耐药性癫痫患者脑组织中的表达

Expression of CDK13 mRNA in the brain tissue of patients with drug-resistant epilepsy

目的:研究耐药性癫痫患者脑组织内依赖细胞周期的蛋白激酶13(Cell cycle-dependent protein kinase 13,CDK13)mRNA的表达。方法:在基因芯片扫描的基础上,运用逆转录-多聚酶链反应(Reverse transcription polymerase chain reaction,RT-PCR)技术检测CDK 13mRNA在难治性癫痢患者脑部的变化。结果:基因芯片扫描显示CDK13 mRNA在耐药性癫痫患者脑部比正常人表达上调2.159倍,RT-PCR检测同样显示CDK13 mRNA在耐药性癫痫患者脑部表达增强,信号明显增高,提示候选基因CDK-13 mRNA在耐药性癫痫患者脑组织中表达上调,RT-PCR与cDNA芯片结果一致。结论:虽然CDK13具体生物功能尚不明确,但我们认为CDK13可能作为重要的调节因子参与了神经元凋亡等分子事件。CDK13mRNA表达上调可能在耐药性癫痫发病机制中起一定作用。

Objective：To study the change of CDK13 mRNA expression in the brain tissue of patients with drug-resistant epilepsy so as to explore the possible mechanisms of the pathogenesis of refractory epilepsy.Methods： The microarray scanning and reverse transcription polymerase chain reaction（RT-PCR） were used to evaluate CDK-13mRNA expression in the brain tissue of 40 refractory epileptic patients and 8 normal controls.Results：As compared with the 8 normal controls,there appeared a significant increase in CDK13 mRNA expression in the patients with drug-resistant epilepsy,with the ratio of cy5/cy3 being 2.159 in microarray scanning（CDK13 mRNA expression in the patients with drug-resistant epilepsy was upregulated by 2.159 times compared with the normal controls.）,and the results of RT-PCR were coincided well with those of the cDNA chip.Conclusion： Further study should be made to clarify the precise function of CDK13.CDK13 may take part in the molecular event in neuronal apoptosis as an important modulator.The up-regulation of CDK13 mRNA expression may play an important role in the epileptogenic mechanisms of drug-resistant epilepsy