强脉冲光对人皮肤MMP-1、MMP-8及TIMP-1表达的影响

Effects of intense pulsed light on matrix metalloproteinases-1,-8 andtissue inhibitor of metalloproteinase-1 from human skin

目的观察强脉冲光照射后人皮肤中基质金属蛋白酶(matrix metalloproteinases,MMPs)及基质金属蛋白酶组织抑制因子(tissue inhibitor of metalloproteinase,TIMP)的表达,探讨强脉冲光嫩肤的分子生物学机制。方法选择皮肤类型为Ⅲ～Ⅳ型志愿者,年龄大于20岁。制定个体化治疗方案,选择恰当的治疗参数,取560 nm/590 nm的滤光片,单/双/三脉冲的不同脉冲方式,脉宽为2.0～4.0 ms,脉冲延迟25～40 ms,能量密度16～20 J/cm2,治疗间隔3周,治疗次数3～4次,于治疗后2～3周在照射部位及非照射部位各切取1块皮肤样本,约5 mm×5 mm×5 mm大小,免疫组化染色,光镜下观察。结果照射后皮肤MMP-1、MMP-8及TIMP-1均有阳性表达,3周时的MMP-1、MMP-8表达弱于2周时的表达,而3周时的TIMP-1表达与2周时相比差异无统计学意义。非照射部位阴性染色。结论强脉冲光可增强人皮肤中MMP-1、MMP-8、TIMP-1的表达,后3者对皮肤重塑起一定的作用。

Objective To analyze quantitatively the alteration of matrix metalloproteinases-1,-8（MMP-1,MMP-8）,tissue inhibitor of metalloproteinase-1（TIMP-1） after radiation with intense pulsed light（IPL） technique in clinic.And to partly explain the treatment mechanism of IPL on sun-damaged human skin and provide a theoretical basis of mechanism,course of therapy and interval time for treatment of IPL on photo aging skin.Methods Volunteers older than 20-years of age whose skin type is Fitzpatrick Ⅲ～Ⅳ were selected.One site of each volunteer was radiated respectively with IPL system at different parameters.Skin specimens were taken at both non-treated and treated locations on days 14 and 21 after IPL irradiation of about the size of 5 mm×5 mm×5 mm.All skin specimens were fixed in formaldehyde fixative buffer.Immunohistochemical staining was performed on each specimen,including observations on changes of MMP-1,MMP-8,TIMP-1 proteins expression after IPL irradiation in order to know their action in dermis remodeling.Results The expressions of MMP-1,MMP-8 and TIMP-1 were detected after treatments.To weeks 2 after treatment.The expressions of MMP-1,MMP-8 became weaker at weeks 3.Expression of TIMP-1 was not significantly different between weeks 2 and 3.There were no expressions in normal skin.Conclusion IPL irradiation could promote the expression of MMP-1,MMP-8 and TIMP-1 in human skin directly.