磷脂酰肌醇蛋白多糖-3在肝细胞癌中的特异性和敏感性以及表达

Specificity and sensitivity and expression of glypican-3 in hepatocellular carcinoma

目的探讨磷脂酰肌醇蛋白多糖-3(glypican-3,GPC3)在高、中/低分化的肝细胞癌(hepatocellular carcinoma,HCC)组中的表达强度、特异性和敏感性。方法挑选首都医科大学附属北京佑安医院2009年12月至2010年9月收治的肝细胞癌病例70例,经过免疫组化染色,观察GPC3在肝细胞癌内以及其周围非肝细胞癌的着色情况和GPC3在所有肝细胞癌病例中的检出率。将70个病例根据癌分化程度分为高、中/低两组。观察GPC3在两组中的着色强度与Ki67在两组中的表达强度是否有相关性。结果①所有肝细胞癌病例的GPC3染色只在肝细胞癌中,肝细胞癌周围非癌组织中均不着色;GPC3在高、中/低两组中总检出率为85%,高分化组中阳性率73.0%,中/低分化组阳性检出率90.2%。②GPC3中强着色度(++、+++)在高分化组中只有2例,占10.5%;在中/低分化组中38例,占74.8%。GPC3在高,中/低分化的肝细胞癌中的表达强度差异有统计学意义(P<0.01)。③Ki67在高分化组中(++、+++)中强度表达是0例,占0%;在中/低分化组中中强度表达是37例,占72.5%,Ki67在高、中/低分化组肝细胞癌中的表达差异有统计学意义(P<0.01)。GPC3在这两组中的着色强度和Ki67在这2组中的的表达强度具有相关性(P<0.01)。结论 GPC3在肝细胞癌中的表达具有高度特异性和敏感性,对肝细胞癌诊断有重要价值。

Objective To investigate specificity,sensitivity and expression of glypican-3（GPC3） in well differentiated,moderately or poorly differentiated hepatocellular carcinoma（HCC） groups Methods Seventy cases with HCC were chosen from our hospital from Dec.2009 to Sep.2010.Expression of GPC3 in HCC as well as in non-HCC surrounding tissue was observed by immunohistochemical-staining and detection rate of GPC3 was calculated.Seventy cases were divided into two groups,well differentiated and moderately/poorly differentiated groups.Correlation between GPC3 and Ki67 were analyzed in two groups.Results ① Staining for GPC3 was positive only in HCC tissues,and was negative in non-HCC surrounding tissue of HCC.Detection rate of GPC3 was 85% in two groups,positive rate 73% in the well differentiated group,90% in the moderately/poorly differentiated group.② Staining intensity was middle/strong for GPC3 in two cases,accounting for 10% in the well differentiated group,38 cases（74.8%） in the mediately/ poorly differentiated group.Expression of GPC3 was significantly different in well differentiated group and moderately/poorly differentiated group（P0.01）.③ In none of the cases the expression level of the Ki67 was moderate and strong（＋ ＋,＋ ＋ ＋）.In 37 cases（72%） of the moderately/poorly differentiated group,the expression was moderately strong.Expression of Ki67 was significantly different in well,moderately/poorly differentiated group（P0.01）.Staining intensity of GPC3 was correlated with expression of Ki67（P0.01）.Conclusion Expression of GPC3 is of the high degree of specificity and sensitivity in HCC.GPC3 may have important value in pathological diagnosis of HCC.