摘要
以灵芝中的三萜类组分(灵芝酸T)为实验药物,考察其对拓扑异构酶Ⅰ活性的影响。结果表明:灵芝酸T能抑制拓扑异构酶Ⅰ活性,且具有浓度依赖性。进一步机理研究表明灵芝酸T可以抑制拓扑异构酶-底物复合物的形成,阻止DNA和酶的相互作用。因此,灵芝酸T属于拓扑异构酶Ⅰ的催化型抑制剂,这可为灵芝中三萜类物质的研究开发提供理论依据。
The triterpenoids components in ganoderma as experimental material were examined on the effect of activity of topoisomerase Ⅰ. The data showed that ganoderic acid T inhibited the activity of topoisomerase Ⅰ behaviors the dose-dependent manner. The inhibition mechanism exploration showed that ganoderic acid T could block the interaction of DNA and topoisomerase Ⅰ by inhibiting the complex formation of the substitute and topoisomerase Ⅰ. The results indicated that ganoderic acid T is a kind of catalysis inhibitor.
出处
《上海应用技术学院学报（自然科学版）》
2011年第2期90-94,共5页
Journal of Shanghai Institute of Technology: Natural Science
基金
上海市教委科研创新项目(09Y2393)
关键词
灵芝酸
拓扑异构酶
抑制作用
ganoderic acid
topoisomerase Ⅰ
inhibition
