HLA-G14bp基因多态性及可溶性HLA-G表达水平与儿童特发性哮喘的关系

The Relationship between the HLA-G 14 bp Polymorphism and Level of Soluable Human Leucucyte Antigen G(sHLA-G) and Atopic Asthmatic in Children

目的检测儿童哮喘患者人白细胞抗原(HLA)-G14bp插入/缺失多态性及血浆可溶性白细胞抗原G(sHLA-G)水平,探讨其与儿童特发性哮喘发病的关系。方法采用聚合酶链反应(PCR)及电泳技术对儿童特发性哮喘患者(85例)和健康体检儿童(98例)进行了HLA-G 14bp插入/缺失多态性基因的检测,用酶联免疫吸附法(ELISA)检测儿童特发性哮喘患者(60例)和健康体检儿童(70例)血浆中可溶性HLA-G(sHLA-G)水平。结果发现HLA-G 14 bp插入/缺失多态性基因及基因型频率分布在儿童特发性哮喘患者和正常对照儿童中均无统计学意义(P均>0.05),儿童特发性哮喘患者中血浆sHLA-G水平(中位数,177.97U/m l)明显高于对照组(中位数,34.92U/m l,P<0.001),哮喘患者的sHLA-G的检测在ROC曲线下面积为0.975,哮喘诊断临界点为158.6U/m l。分析患者及正常对照儿童血浆sHLA-G水平在HLA-G 14bp插入/缺失多态性基因型频率分布中的差异,发现均无统计学意义(P均>0.05),患者血浆sHLA-G水平与过敏原的种类、总IgE及特异性IgE(sIgE)的含量也无相关性(P均>0.05)。结论 HLA-G 14 bp插入/缺失多态性基因和儿童特发性哮喘的易感性无关,而sHLA-G可能作为儿童特发性哮喘的一个生物标志物,在儿童哮喘的发病机制中发挥重要的作用。

Objective To determine the humun leukocyte antigen - G（ HLA - G） 14bp insertion/deletion polymorphism and plasma soluble HLA -G（ sHLA -G） levels in patients with childhood atopic asthma, and to analyze its association with the pathogenesis of childhood atopic asthma. Methods Polymerase chain reaction（PCR） was performed to detect the genotypes of HLA - G 14bp polymorphism in childhood atopic asthma（ n = 85 ） and controls（ n = 98 ）. Soluble HLA - G （ sHLA - G） levels were determined by enzyme - linked immunosorbent assay in childhood atopic asthma（ n = 60 ） and normal controls （ n = 70 ）. Results HLA - G 14bp insertion/deletion polymorphism and its frequency of genotype were of no significant difference between the asthmatic patients and normal controls（ P 〉 0.05 ）. Plas- ma soluble HLA -G（ sHLA- G） in atopic asthma patients（ median, 177.97U/ml） was dramatically higher compared with that of the normal controls （ median,34.92U/ml ;P 〈 O. 001 ）. The area under ROC curve for sHLA - G was O. 975 for atopic asthma patients versus normal controls, and the cut - off value was 169.02U/ml. For luther studies,both in asthmatic children and normal controls, sHLA - G expression was not significantly related to the genotypes of the HLA - G 14 - bp insertlon/deletion polymorphism （P 〉 0.05）. No significant association was observed between the plasma sHLA - G levels and total IgE,allergen specific IgE scores,and the types of allergen in the study （P 〉 0.05）. Conclusion There was no association between HLA - G 14 bp polymorphism gene and the suscepbility of childhood atopic asthma. However,sHLA- G which plays an important role in the pathogenesis of children asthma can be an potential biomarker for the atopie asthma patients.