HER3表达与宫颈鳞状细胞癌局部血管生成和浸润转移及预后的关系

Relationship between the expressions of HER3 and regional angiogenesis, invasion, metastasis and prognosis in cervical squamous cell carcinoma

目的 探讨HER3在宫颈鳞状细胞癌组织的表达及其临床意义.方法 采用免疫组化SP法检测HER3在28例宫颈正常上皮（NCE）、36例宫颈上皮内瘤变（CIN）和68例宫颈鳞状细胞癌（SCC）组织的表达,并检测其中Ki-67标记的增殖指数（PI）和CD34标记的微血管密度（MVD）.结果 HER3在NCE、CIN及SCC组的阳性表达率分别为10.7%（3/28）、22.2%（8/36）及54.4%（37/68）.HER3在SCC组的阳性表达率均高于CIN和NCE组（P＜0.05）.HER3在SCC组表达分别与PI及MVD显著正相关（PI：r=0.375,P=0.002;MVD：r=0.273,P=0.024）.HER3在SCC组表达分别与FIGO分期及组织学分级有关（P＜0.05）,而与患者年龄、有否盆腔淋巴结转移、脉管浸润、间质浸润深度及生存率无关（P＞0.05）.FIGO分期为Ⅱ期及组织学分级为Ⅲ级者,其HER3的阳性表达率分别显著高于FIGO分期为Ⅰ期及组织学分级在Ⅱ级以内者（P＜0.05）.结论 HER3过度表达可能上调宫颈鳞癌细胞增殖及间质血管新生,可能促进宫颈鳞癌病程进展.

Objective To investigate the expressions of HER3 in invasive squamous cell carcinoma of cervix and the clinical significance. Methods The expressions of HER3, Ki-67 and CD34 protein in 28 cases of normal cervical epithelium（NCE）, 36 cases of cervical intraepithelial neoplasm（CIN） and 68 cases of squamous cell carcinoma of cervix （SCC） were detected by immunohistochemistry streptavidin-perosidase （SP） method. Results The positive expression rates of HER3 in NCE, CIN and SCC were 10.7%, 22.2% and 54.4%, respectively. The positive expression rates of HER3 increased remarkably from NCE and CIN to SCC （P 〈 0. 05）. The expression of HER3 in SCC was significantly positive correlated with PI and MVD（for PI： r =0.375, P=0. 002; for MVD： r=0.273, P =0. 024）. The expression of HER3 in SCC was related with FIGO staging and histological grading（P 〈 0.05）, but was not related with patients age, pelvic lymph node metastasis, depth of stroma infiltration, intravascular infiltration and survival rate（P 〉0.05）. In the cases with FIGO staging Ⅱ and histological grading Ⅲ, the positive expression rate of HER3 was significantly higher than that in the cases without them （P 〈 0. 05）. Conclusion The over-expression of HER3 may lead to up regulation of tumor angiogenesis and cancer cell proliferation in SCC,and may promote progress of SCC.