Scu-PA/t-PA基因cDNA在小鼠体内的表达

Direct Scu-PA/t-PA cDNA Injection into Mouse Muscle and Expression in Vivo

将SCC－PA／t－PA两种基因CDNA所构建的表达载体PSV_2ScUPA，pcDNA－ScUPA及pcDNA－tPA混合后注入小鼠骨骼肌，观察了基因混合注射在小鼠体内的表述情况。体外凝血试验结果显示．质拉注射后第3天凝血时间开始延长，约两周后凝血时间延长幅度最大。第18天体内诱导产生DIC，体外投血时间试验组仍较空白对照组延长一倍以上．抗凝效果明显。相等基因水平下．混合基因注射表达水平和单独基因注射没有差别。减半注射两种混合基因我体．其抗凝效果与不减量单独注射和混合注射效果相同。

Two typePA (Scu-PA and t-PA)expressed in mouse musclc were obscrvcd by dircc[ lnJcct1on ofpcDNA,-ScuPA and pcDNA,-tPA Vcctors,as wcll as the mixture of pSV,-ScuPA, pcDN A,-ScuPA and pcD-NA,~tPA into the musclc? The result demonstratcd that the prolongation of blood clottlng timc could bc dls-cerned at the 3rd day after the injection of the rccombinant Vectors,and thc peak occurred two weeks afterinjecting. On an average,the blood coagulation 11n1c prolonged for 5 minutes and there was no diffcrence hc-tween the cxprcssion levels of single vector and mixture vectors. Disscminated lnlravascular coagulation(DIC) was induced at the 18th day,the cffect of autl-clotting remain observed.