Ⅰ类整合子介导的铜绿假单胞菌耐药性分析

Analysis of drug resistance mediated by class Ⅰ integron in Pseudomonas aeruginosa

目的检测铜绿假单胞菌(PA)的Ⅰ类整合子,探讨其与细菌耐药性的关系。方法以VITEK-AMS微生物自动分析仪进行细菌鉴定和药敏试验;PCR扩增Ⅰ类整合酶基因,电泳分析PCR扩增产物。结果 116株PA中,Ⅰ类整合子检出率为25.9%(30/116)。Ⅰ类整合子阳性PA对氨基糖苷类、喹诺酮类及头孢菌素类药物的耐药率较高。Ⅰ类整合子阳性PA多药耐药率为80.0%,高于阴性菌株的17.4%(P<0.05)。结论Ⅰ类整合子与PA耐药性的形成和耐药基因的播散密切相关。

Objective To explore the relationship between the class Ⅰ integron and drug resistance in Pseudomonas aeruginosa（PA）.Methods Identification and susceptibility test of PA were performed on VITEK-AMS automatic bacterial test systems.Class Ⅰ integron gene in PA was amplified by polymerase chain reaction and the productions were examined by electrophoresis.Results In all 116 strains of PA,25.9%（30/116） were positive with class Ⅰ integron.The multi-drug resistance rate of PA,positive with class Ⅰ integron,was 80.0%,higher the 17.4% of PA,negative with class Ⅰ integron（P〈0.05）.Conclusion Class Ⅰ integron could be closely related with the emergence of drug resistance and the dissemination of drug resistance genes in PA