某地区2010年手足口病患者肠道病毒RNA实时荧光逆转录PCR检测结果分析

Detection enterovirus RNA of poctients with hand-foot-mouth disease in 2010 in certain area by real time fluorescent quantitative PCR method

目的用实时荧光定量RT-PCR技术检测惠州市2010年手足口病患者肠道病毒RNA,了解手足口病病例肠道病毒的感染情况,为制定预防和控制肠道病毒感染策略提供依据。方法采集2010年1～12月本院送检的1712例手足口病患者的粪便、肛拭子、咽拭子和脑脊液等标本5849份,应用荧光RT-PCR技术检测样本中的肠道病毒(EV)、肠道病毒71型(EV71)、柯萨奇病毒A组16型(CA16)。结果男性及女性EV、EV71和CA16感染率分别为68.15%、43.61%、5.56%和61.23%、37.03%、5.70%;共检出1123份EV阳性标本,705份EV71阳性标本,96份CA16阳性标本,阳性率分别为60.28%、33.35%和5.13%;4～6月和7～9月是手足口病发病高峰期,感染肠道病毒EV、EV71型、CA16型人数分别占所有肠道病毒EV、EV71、CA16型感染者的36.33%、49.50%、42.70%和42.56%、38.72%、40.63%;0～4岁的儿童是手足口病的高发人群,占所有患者的85.46%。结论惠州市2010年手足口病的病原以肠道病毒71型为主,夏秋季节是手足口病发病高峰期,0～4岁儿童是感染肠道病毒的高危人群。

Objective To detect enterovirus RNA of hand-foot-mouth disease（HFMD） of the patients in Huizhou city in 2010 by real time fluorescent quantitative PCR method and understand the genotype of enterovirus of HFMD so as to provide a scientific basis for preventing and controling HFMD.Methods A total of 5 849 samples including feces,anal swab,throat swabs,Herpes fluid and other types from 1 712 patients were collected from January to December in 2010.Nucleic acids of three types of viruses of enterovirus（EV）,enter virus 71（EV71） and Coxasckie virus A16（CA16） were detected by real time fluorescent quantitative PCR method.Results The infectious rate of EV,EV71 and CA16 in boy and girl were 68.15%,43.61%,5.56% and 61.23%,37.03%,5.70% respectively.In 5 849 samples from the patients,1 123 were EV positive,705 were EV71 positive and 96 were CA16 positive;The positive rates of EV,EV71 andCA16 were 60.28%,33.35% and 5.13% respectively.The infant case from 0-4 years old was the highest one,which accounted for 85.46%.The outbreak smostly happened during April to June and July to September.Its infectious rates of EV,EV71 and CA16 were 36.33%,49.50%,42.70% and 42.56%,38.72%,40.63% respectively.Conclusion EV71 is the primary pathogen of HFMD in Huizhou city in 2010.Its outbreaks mostly happened in summer and autumn.HFMD tends to occur in younger children from 0-4 years old.