CyFlow Space流式细胞仪CD34^+细胞绝对计数分析

Research on absolute enumeration of CD34^+ cells detected by CyFlow Space flow cytometry

目的比较在Partec CyFlow Space流式细胞仪上采用不同设门方法计数CD34+造血干细胞结果的差异。方法采集经骨髓动员的患者外周静脉血标本30例,在分别采用同型对照设门法和国际血液治疗与移植工程协会(ISHAGE)推荐设门法设置流式细胞仪后进行标本测定。结果两种设门法标本计数结果具有相关性(r=0.990),但ISHAGE设门法的计数结果低于同型对照设门法(P<0.05)。回收试验显示,ISHAGE设门法计数不同细胞浓度标本的预期值与回收值的相关系数为0.9989;对3种细胞浓度重复检测的变异系数为2.2%～3.8%。结论两种设门法计数CD34+细胞的相关性较好,但ISHAGE设门法更能反映造血干细胞的特点,具有良好的准确度和精密度,且无需同型对照抗体,节约试剂,更适合临床常规使用。

Objective To compare the difference of CD34^＋ cells counts detected by Partec CyFlow Space flow cytometry using two different gating strategies.Methods 30 peripheral blood samples were collected from patients after being treated by bone marrow mobilization,and measured by using ISHAGE gating strategy,recommended by the International Society for Hematotherapy and Graft Engineering,and isotype gating strategy.Results There was fine correlation between the results detected by the two methods（r=0.990）,but the detection result of ISHAGE gating was lower than that of isotype gating strategy（P〈0.05）.Recovery test indicated that the correlation coefficient between expected and tested value was 0.9989,with the coefficient of variation was 2.2% to 3.8% when three samples with different cell concentration were detected by using ISHAGE gating strategy.Conclusion There could be fine correlation between the results detected by the two gating strategies,but ISHAGE gating strategy is more accurate,with lower cost,and more suitable for clinical laboratory application.