CCN2基因在结直肠癌中的表达及其临床意义的研究

The study of CCN2 gene expression in colorectal cancer and its clinic pathological significance

目的研究CCN2在结直肠癌中的表达及其与临床、病理指标的关系。方法用免疫组化方法检测结直肠癌60例，结直肠癌肝转移、结直肠腺瘤、癌旁组织及正常结直肠粘膜组织各30例样本中CCN2的表达。结果（1）在结直肠癌和结直肠癌肝转移组织中CCN2蛋白均表现为高表达，分别为75．00％和70．00％；而在结直肠腺瘤、癌旁组织、正常结直肠粘膜组织表达低，分别为23．33％、6．67％、0．00％；差异有极显著性（尸〈0．01l（2）结直肠癌组织中CCN2表达与肿瘤的TNM分期、淋巴结转移及5年生存率作比较差异有显著性（P〈0．05）。结论CCN2基因可能作为结直肠癌的一个癌基因并参与结直肠癌的发生、发展，可考虑作为诊断结直肠癌或判断结直肠癌预后的指标。

Objective To research the CCN2 expression in colorectal carcinoma and its correlation with clinical pathological parameters. Methods Expression of CCN2 protein in several kinds of colorectal tissue was examined with immunohistochemistry. Results （1）CCN2 protein had high-expression in colorectal carcinoma and distant metastatic tissues in live~; the expressions were 75.00% and 70.00% respectively. And adenoma of colorectum, adjacent non-cancerous tissue, and mucosa tissue of normal rectum were low-expression of CCN2 protein; the expressions were 23.33%, 6.67%, and 0.00% respectively. The discrepancy had statistical significance （P〈 0.001）. （2）CCN2 protein expression had significant correlation with neoplasm phases, five-year survival rate, and lymph node metastasis （P 〈 0.05）. Conclusion CCN2 may be identified as an oncogene and play an important role on the occurrence and development of colorectal carcinoma. CCN2 may act as an effective indicator for diagnosing colorectal carcinoma and as a factor for assessing its prognosis.