聚乙烯醇颗粒的急性毒性实验

Experiment of acute toxicity of polyvinyl alcohol particles

将30只新西兰大白兔分成6组:低、较低剂量、中、较高剂量、高剂量PVA组、对照组组(1.0 mg.kg～1,1.6 mg.kg～1,2.0 mg.kg～1,2.5 mg.kg～1,5.0 mg.kg～1,40%泛影葡胺2ml/只),经皮穿刺肝动脉给药,连续观察给药后的急性毒性表现,共14d,并检测血液生化指标,进行病理组织学检查,LD50采用Bliss法计算。PVA肝动脉给药的LD50为1.94(1.65～2.28)mg.kg–1。与对照组新西兰大白兔的血生化指标相比,1～3d各组新西兰大白兔血中ALT、AST、ALP、TbiL、DbiL、BCr、BUN明显升高,7～14d上述指标逐渐恢复;TP、ALB、γ～GT则变化不大。病理组织学检查则表明均有肝、胆囊组织损伤和脾、胃、小肠、肾等异位栓塞。

Will only New Zealand big white rabbit divide into 6 groups：low,lower doses,medium and a higher dose,high doses of PVA group,controlled qol（1.0 mg·kg-1,1.6 mg·kg-1,2.0 mg·kg-1,2.5 mg·kg-1,5.0 mg·kg and 40%2ml/meglumini diatrozoas only）,percutaneous hepatic artery to medicine,continuous observation after the administration of acute toxicity performance,14d,and testing blood biochemical indicators,histologic examination.By Bliss method to calculate the LD50.Hepatic artery dosing of PVA for since（LD50,1.65～2.28 kg-1）mg.And controls the blood of New Zealand big white rabbit biochemical indexes,1～3d group than in New Zealand big white rabbit blood AST,ALP,ALT,DbiL TbiL,BCr,BUN increased significantly,7-14d above index recover gradually;TP,ALB,gamma～GT is little change.Histopathology shows all have liver,gallbladder and spleen,stomach tissue damage,small intestine,kidney and other ectopic embolism.