弓形虫病的分子诊断

Molecular diagnosis of toxoplasmosis

近年来,弓形虫病分子诊断技术发展较快,本文介绍核酸分子杂交、聚合酶链反应(PCR)常用的弓形虫基因序列,以及常规PCR、巢式PCR、实时定量PCR、免疫PCR、原位PCR、环介导等温扩增等分子生物学技术在弓形虫病诊断中的研究与应用。

This paper reviews the domestic and foreign literatures regarding molecular diagnosis of toxoplasmosis.The nucleic acid molecule hybridization and Toxoplasma gondii gene sequences for polymerase chain reaction（PCR）,common and convention-al PCR,nested PCR,real time quantitative PCR,immune PCR,in-situ PCR,loop mediated isothermal amplification technology are introduced.