卵巢透明细胞癌基因表达谱初探

Primary exploration for gene expression profile of ovarian clear cell carcinoma

目的初步建立卵巢透明细胞癌基因表达谱,探讨透明细胞癌和浆液性囊腺癌之间的差异表达基因。方法用一步法抽提8例卵巢透明细胞癌组织和8例卵巢浆液性囊腺癌组织的总RNA,反转录合成cDNA探针的同时以Cy3、Cy5分别标记透明细胞癌和浆液性囊腺癌。两种探针等量混合后与含有14 000多条人类全基因的表达谱芯片杂交。经洗片、扫描和图像处理,分析卵巢透明细胞癌和浆液性囊腺癌之间基因表达谱差异。结果卵巢透明细胞癌和卵巢浆液性囊腺癌基因表达谱比较,差异2倍以上基因共有82个,表达上调的有25个,表达下调的有57个。其中有功能的表达上调基因9个,表达下调基因12个。结论建立的卵巢透明细胞癌和浆液性囊腺癌差异表达基因谱,有助于深入研究卵巢透明细胞癌分子生物学特点。

Objective To investigate genes differential expression between the ovarian clear cell carcinoma and the ovarian cystadenocarcinoma by gene expression profiles. Methods The total RNA of 8 clear cell carcinomas and 8 serous cystadenocarcinomas were isolated by using TRIzol method, then reversely transcripted them to cDNA with Cy3 and Cy5 labeling respectively. Equal quantity of labeled cDNA were mixed and then hybridized in the cDNA microarray with more than 14 000 unigene. After washing, scanning and image processing, the difference of gene expression profiles between clear cell carcinoma and serous cystadenocarcinoma was analyzed. Results Totally 82 genes had more than two fold different expression, the up-regulated and down-regulated genes were 25 and 57 respectively. Nine genes up-regulated and 12 genes down-regulated have identified fimction. Conclusion Establishment of the gene expression profile between two type ovarian carcinomas contributes to the exploration of the molecular biologic characteristic of the ovarian clear cell carcinoma.